Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation

The marine roseobacter-clade affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic clim...

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Autores principales: Silvano, Eleonora, Yang, Mingyu, Wolterink, Mathias, Giebel, Helge-Ansgar, Simon, Meinhard, Scanlan, David J., Zhao, Yanlin, Chen, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779409/
https://www.ncbi.nlm.nih.gov/pubmed/33408696
http://dx.doi.org/10.3389/fmicb.2020.552135
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author Silvano, Eleonora
Yang, Mingyu
Wolterink, Mathias
Giebel, Helge-Ansgar
Simon, Meinhard
Scanlan, David J.
Zhao, Yanlin
Chen, Yin
author_facet Silvano, Eleonora
Yang, Mingyu
Wolterink, Mathias
Giebel, Helge-Ansgar
Simon, Meinhard
Scanlan, David J.
Zhao, Yanlin
Chen, Yin
author_sort Silvano, Eleonora
collection PubMed
description The marine roseobacter-clade affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodeling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), RCA bacteria synthesize a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation.
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spelling pubmed-77794092021-01-05 Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation Silvano, Eleonora Yang, Mingyu Wolterink, Mathias Giebel, Helge-Ansgar Simon, Meinhard Scanlan, David J. Zhao, Yanlin Chen, Yin Front Microbiol Microbiology The marine roseobacter-clade affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodeling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), RCA bacteria synthesize a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779409/ /pubmed/33408696 http://dx.doi.org/10.3389/fmicb.2020.552135 Text en Copyright © 2020 Silvano, Yang, Wolterink, Giebel, Simon, Scanlan, Zhao and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Silvano, Eleonora
Yang, Mingyu
Wolterink, Mathias
Giebel, Helge-Ansgar
Simon, Meinhard
Scanlan, David J.
Zhao, Yanlin
Chen, Yin
Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title_full Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title_fullStr Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title_full_unstemmed Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title_short Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
title_sort lipidomic analysis of roseobacters of the pelagic rca cluster and their response to phosphorus limitation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779409/
https://www.ncbi.nlm.nih.gov/pubmed/33408696
http://dx.doi.org/10.3389/fmicb.2020.552135
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