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Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function

Imperatorin (IMP) exhibits a variety of pharmacological properties, including antioxidant, anti-inflammatory, antibacterial, anti-cancer, and anti-hypertension activities. However, its effects on animal reproduction systems, especially oocyte development, maturation, and aging are not yet clear. In...

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Autores principales: Luo, Dan, Zhang, Jia-bao, Li, Sheng-peng, Liu, Wen, Yao, Xue-rui, Guo, Hao, Jin, Zhe-long, Jin, Yong-xun, Yuan, Bao, Jiang, Hao, Kim, Nam-Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779485/
https://www.ncbi.nlm.nih.gov/pubmed/33409275
http://dx.doi.org/10.3389/fcell.2020.592433
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author Luo, Dan
Zhang, Jia-bao
Li, Sheng-peng
Liu, Wen
Yao, Xue-rui
Guo, Hao
Jin, Zhe-long
Jin, Yong-xun
Yuan, Bao
Jiang, Hao
Kim, Nam-Hyung
author_facet Luo, Dan
Zhang, Jia-bao
Li, Sheng-peng
Liu, Wen
Yao, Xue-rui
Guo, Hao
Jin, Zhe-long
Jin, Yong-xun
Yuan, Bao
Jiang, Hao
Kim, Nam-Hyung
author_sort Luo, Dan
collection PubMed
description Imperatorin (IMP) exhibits a variety of pharmacological properties, including antioxidant, anti-inflammatory, antibacterial, anti-cancer, and anti-hypertension activities. However, its effects on animal reproduction systems, especially oocyte development, maturation, and aging are not yet clear. In this study, the effects of IMP on oocyte development and aging as well as the underlying molecular mechanisms were explored. Oocytes were cultured for an additional 24 h for aging. Results revealed that the blastocyst formation and hatching rates of embryos, which were parthenogenetically activated aged oocytes, were significantly increased with IMP treatment (40 μM). Simultaneously, well-distributed cortical granules but no significant difference in zona pellucida hardness were observed after IMP treatment. During this stage, intracellular reactive oxygen species, apoptosis, and autophagy levels were decreased, while mitochondrial membrane potential, glutathione level, and activity of superoxide dismutase and catalase were increased. IMP-treated aged oocytes also showed significantly higher expression of MOS, CCNB1, BMP15, and GDF9 than non-IMP-treated aged oocytes although their levels were still lower than those in the fresh oocytes. These results suggest that IMP can effectively ameliorate the quality of aged porcine oocytes by reducing oxidative stress and protecting mitochondrial function.
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spelling pubmed-77794852021-01-05 Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function Luo, Dan Zhang, Jia-bao Li, Sheng-peng Liu, Wen Yao, Xue-rui Guo, Hao Jin, Zhe-long Jin, Yong-xun Yuan, Bao Jiang, Hao Kim, Nam-Hyung Front Cell Dev Biol Cell and Developmental Biology Imperatorin (IMP) exhibits a variety of pharmacological properties, including antioxidant, anti-inflammatory, antibacterial, anti-cancer, and anti-hypertension activities. However, its effects on animal reproduction systems, especially oocyte development, maturation, and aging are not yet clear. In this study, the effects of IMP on oocyte development and aging as well as the underlying molecular mechanisms were explored. Oocytes were cultured for an additional 24 h for aging. Results revealed that the blastocyst formation and hatching rates of embryos, which were parthenogenetically activated aged oocytes, were significantly increased with IMP treatment (40 μM). Simultaneously, well-distributed cortical granules but no significant difference in zona pellucida hardness were observed after IMP treatment. During this stage, intracellular reactive oxygen species, apoptosis, and autophagy levels were decreased, while mitochondrial membrane potential, glutathione level, and activity of superoxide dismutase and catalase were increased. IMP-treated aged oocytes also showed significantly higher expression of MOS, CCNB1, BMP15, and GDF9 than non-IMP-treated aged oocytes although their levels were still lower than those in the fresh oocytes. These results suggest that IMP can effectively ameliorate the quality of aged porcine oocytes by reducing oxidative stress and protecting mitochondrial function. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779485/ /pubmed/33409275 http://dx.doi.org/10.3389/fcell.2020.592433 Text en Copyright © 2020 Luo, Zhang, Li, Liu, Yao, Guo, Jin, Jin, Yuan, Jiang and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Luo, Dan
Zhang, Jia-bao
Li, Sheng-peng
Liu, Wen
Yao, Xue-rui
Guo, Hao
Jin, Zhe-long
Jin, Yong-xun
Yuan, Bao
Jiang, Hao
Kim, Nam-Hyung
Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title_full Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title_fullStr Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title_full_unstemmed Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title_short Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function
title_sort imperatorin ameliorates the aging-associated porcine oocyte meiotic spindle defects by reducing oxidative stress and protecting mitochondrial function
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779485/
https://www.ncbi.nlm.nih.gov/pubmed/33409275
http://dx.doi.org/10.3389/fcell.2020.592433
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