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Global View on the Cytokinin Regulatory System in Potato

Cytokinins (CKs) were earlier shown to promote potato tuberization. Our study aimed to identify and characterize CK-related genes which constitute CK regulatory system in the core potato (Solanum tuberosum) genome. For that, CK-related genes were retrieved from the sequenced genome of the S. tuberos...

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Autores principales: Lomin, Sergey N., Myakushina, Yulia A., Kolachevskaya, Oksana O., Getman, Irina A., Savelieva, Ekaterina M., Arkhipov, Dmitry V., Deigraf, Svetlana V., Romanov, Georgy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779595/
https://www.ncbi.nlm.nih.gov/pubmed/33408733
http://dx.doi.org/10.3389/fpls.2020.613624
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author Lomin, Sergey N.
Myakushina, Yulia A.
Kolachevskaya, Oksana O.
Getman, Irina A.
Savelieva, Ekaterina M.
Arkhipov, Dmitry V.
Deigraf, Svetlana V.
Romanov, Georgy A.
author_facet Lomin, Sergey N.
Myakushina, Yulia A.
Kolachevskaya, Oksana O.
Getman, Irina A.
Savelieva, Ekaterina M.
Arkhipov, Dmitry V.
Deigraf, Svetlana V.
Romanov, Georgy A.
author_sort Lomin, Sergey N.
collection PubMed
description Cytokinins (CKs) were earlier shown to promote potato tuberization. Our study aimed to identify and characterize CK-related genes which constitute CK regulatory system in the core potato (Solanum tuberosum) genome. For that, CK-related genes were retrieved from the sequenced genome of the S. tuberosum doubled monoploid (DM) Phureja group, classified and compared with Arabidopsis orthologs. Analysis of selected gene expression was performed with a transcriptome database for the S. tuberosum heterozygous diploid line RH89-039-16. Genes responsible for CK signaling, biosynthesis, transport, and metabolism were categorized in an organ-specific fashion. According to this database, CK receptors StHK2/3 predominate in leaves and flowers, StHK4 in roots. Among phosphotransmitters, StHP1a expression largely predominates. Surprisingly, two pseudo-phosphotransmitters intended to suppress CK effects are hardly expressed in studied organs. Among B-type RR genes, StRR1b, StRR11, and StRR18a are actively expressed, with StRR1b expressing most uniformly in all organs and StRR11 exhibiting the highest expression in roots. By cluster analysis four types of prevailing CK-signaling chains were identified in (1) leaves and flowers, StHK2/3→StHP1a→StRR1b/+; (2) shoot apical meristems, stolons, and mature tubers, StHK2/4→StHP1a→StRR1b/+; (3) stems and young tubers, StHK2/4→StHP1a→StRR1b/11/18a; and (4) roots and tuber sprouts, StHK4→StHP1a→StRR11/18a. CK synthesis genes StIPT3/5 and StCYP735A are expressed mainly in roots followed by tuber sprouts, but rather weakly in stolons and tubers. By contrast, CK-activation genes StLOGs are active in stolons, and StLOG3b expression is even stolon-confined. Apparently, the main CK effects on tuber initiation are realized via activity of StLOG1/3a/3b/7c/8a genes in stolons. Current advances and future directions in potato research are discussed.
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spelling pubmed-77795952021-01-05 Global View on the Cytokinin Regulatory System in Potato Lomin, Sergey N. Myakushina, Yulia A. Kolachevskaya, Oksana O. Getman, Irina A. Savelieva, Ekaterina M. Arkhipov, Dmitry V. Deigraf, Svetlana V. Romanov, Georgy A. Front Plant Sci Plant Science Cytokinins (CKs) were earlier shown to promote potato tuberization. Our study aimed to identify and characterize CK-related genes which constitute CK regulatory system in the core potato (Solanum tuberosum) genome. For that, CK-related genes were retrieved from the sequenced genome of the S. tuberosum doubled monoploid (DM) Phureja group, classified and compared with Arabidopsis orthologs. Analysis of selected gene expression was performed with a transcriptome database for the S. tuberosum heterozygous diploid line RH89-039-16. Genes responsible for CK signaling, biosynthesis, transport, and metabolism were categorized in an organ-specific fashion. According to this database, CK receptors StHK2/3 predominate in leaves and flowers, StHK4 in roots. Among phosphotransmitters, StHP1a expression largely predominates. Surprisingly, two pseudo-phosphotransmitters intended to suppress CK effects are hardly expressed in studied organs. Among B-type RR genes, StRR1b, StRR11, and StRR18a are actively expressed, with StRR1b expressing most uniformly in all organs and StRR11 exhibiting the highest expression in roots. By cluster analysis four types of prevailing CK-signaling chains were identified in (1) leaves and flowers, StHK2/3→StHP1a→StRR1b/+; (2) shoot apical meristems, stolons, and mature tubers, StHK2/4→StHP1a→StRR1b/+; (3) stems and young tubers, StHK2/4→StHP1a→StRR1b/11/18a; and (4) roots and tuber sprouts, StHK4→StHP1a→StRR11/18a. CK synthesis genes StIPT3/5 and StCYP735A are expressed mainly in roots followed by tuber sprouts, but rather weakly in stolons and tubers. By contrast, CK-activation genes StLOGs are active in stolons, and StLOG3b expression is even stolon-confined. Apparently, the main CK effects on tuber initiation are realized via activity of StLOG1/3a/3b/7c/8a genes in stolons. Current advances and future directions in potato research are discussed. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779595/ /pubmed/33408733 http://dx.doi.org/10.3389/fpls.2020.613624 Text en Copyright © 2020 Lomin, Myakushina, Kolachevskaya, Getman, Savelieva, Arkhipov, Deigraf and Romanov. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lomin, Sergey N.
Myakushina, Yulia A.
Kolachevskaya, Oksana O.
Getman, Irina A.
Savelieva, Ekaterina M.
Arkhipov, Dmitry V.
Deigraf, Svetlana V.
Romanov, Georgy A.
Global View on the Cytokinin Regulatory System in Potato
title Global View on the Cytokinin Regulatory System in Potato
title_full Global View on the Cytokinin Regulatory System in Potato
title_fullStr Global View on the Cytokinin Regulatory System in Potato
title_full_unstemmed Global View on the Cytokinin Regulatory System in Potato
title_short Global View on the Cytokinin Regulatory System in Potato
title_sort global view on the cytokinin regulatory system in potato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779595/
https://www.ncbi.nlm.nih.gov/pubmed/33408733
http://dx.doi.org/10.3389/fpls.2020.613624
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