Cargando…

Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.

Capsaicinoids are naturally specialized metabolites in pepper and are the main reason that Capsicum fruits have a pungent smell. During the synthesis of capsaicin, MYB transcription factors play key regulatory roles. In particular, R2R3-MYB subfamily genes are the most important members of the MYB f...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jin, Liu, Yi, Tang, Bingqian, Dai, Xiongze, Xie, Lingling, Liu, Feng, Zou, Xuexiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779616/
https://www.ncbi.nlm.nih.gov/pubmed/33408738
http://dx.doi.org/10.3389/fgene.2020.598183
_version_ 1783631370438836224
author Wang, Jin
Liu, Yi
Tang, Bingqian
Dai, Xiongze
Xie, Lingling
Liu, Feng
Zou, Xuexiao
author_facet Wang, Jin
Liu, Yi
Tang, Bingqian
Dai, Xiongze
Xie, Lingling
Liu, Feng
Zou, Xuexiao
author_sort Wang, Jin
collection PubMed
description Capsaicinoids are naturally specialized metabolites in pepper and are the main reason that Capsicum fruits have a pungent smell. During the synthesis of capsaicin, MYB transcription factors play key regulatory roles. In particular, R2R3-MYB subfamily genes are the most important members of the MYB family and are critical candidate factors in capsaicinoid biosynthesis. The 108 R2R3-MYB genes in pepper were identified in this study and all are shown to have two highly conserved MYB binding domains. Phylogenetic and structural analyses clustered CaR2R3-MYB genes into seven groups. Interspecies collinearity analysis found that the R2R3-MYB family contains 16 duplicated gene pairs and the highest gene density is on chromosome 00 and 03. The expression levels of CaR2R3-MYB differentially expressed genes (DEGs) and capsaicinoid-biosynthetic genes (CBGs) in fruit development stages were obtained via RNA-seq and quantitative polymerase chain reaction (qRT-PCR). Co-expression analyses reveal that highly expressed CaR2R3-MYB genes are co-expressed with CBGs during early stages of pericarp and placenta development processes. It is speculated that six candidate CaR2R3-MYB genes are involved in regulating the synthesis of capsaicin and dihydrocapsaicin. This study is the first systematic analysis of the CaR2R3-MYB gene family and provided references for studying their molecular functions. At the same time, these results also laid the foundation for further research on the capsaicin characteristics of CaR2R3-MYB genes in pepper.
format Online
Article
Text
id pubmed-7779616
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77796162021-01-05 Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L. Wang, Jin Liu, Yi Tang, Bingqian Dai, Xiongze Xie, Lingling Liu, Feng Zou, Xuexiao Front Genet Genetics Capsaicinoids are naturally specialized metabolites in pepper and are the main reason that Capsicum fruits have a pungent smell. During the synthesis of capsaicin, MYB transcription factors play key regulatory roles. In particular, R2R3-MYB subfamily genes are the most important members of the MYB family and are critical candidate factors in capsaicinoid biosynthesis. The 108 R2R3-MYB genes in pepper were identified in this study and all are shown to have two highly conserved MYB binding domains. Phylogenetic and structural analyses clustered CaR2R3-MYB genes into seven groups. Interspecies collinearity analysis found that the R2R3-MYB family contains 16 duplicated gene pairs and the highest gene density is on chromosome 00 and 03. The expression levels of CaR2R3-MYB differentially expressed genes (DEGs) and capsaicinoid-biosynthetic genes (CBGs) in fruit development stages were obtained via RNA-seq and quantitative polymerase chain reaction (qRT-PCR). Co-expression analyses reveal that highly expressed CaR2R3-MYB genes are co-expressed with CBGs during early stages of pericarp and placenta development processes. It is speculated that six candidate CaR2R3-MYB genes are involved in regulating the synthesis of capsaicin and dihydrocapsaicin. This study is the first systematic analysis of the CaR2R3-MYB gene family and provided references for studying their molecular functions. At the same time, these results also laid the foundation for further research on the capsaicin characteristics of CaR2R3-MYB genes in pepper. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779616/ /pubmed/33408738 http://dx.doi.org/10.3389/fgene.2020.598183 Text en Copyright © 2020 Wang, Liu, Tang, Dai, Xie, Liu and Zou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Jin
Liu, Yi
Tang, Bingqian
Dai, Xiongze
Xie, Lingling
Liu, Feng
Zou, Xuexiao
Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title_full Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title_fullStr Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title_full_unstemmed Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title_short Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
title_sort genome-wide identification and capsaicinoid biosynthesis-related expression analysis of the r2r3-myb gene family in capsicum annuum l.
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779616/
https://www.ncbi.nlm.nih.gov/pubmed/33408738
http://dx.doi.org/10.3389/fgene.2020.598183
work_keys_str_mv AT wangjin genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT liuyi genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT tangbingqian genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT daixiongze genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT xielingling genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT liufeng genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml
AT zouxuexiao genomewideidentificationandcapsaicinoidbiosynthesisrelatedexpressionanalysisofther2r3mybgenefamilyincapsicumannuuml