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Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease
Extracts from Holothuria scabra (HS) have been shown to possess anti-inflammation, anti-oxidant and anti-cancer activities. More recently, it was shown to have neuroprotective potential in Caenorhabditis elegans PD model. Here, we assessed whether HS has neuroprotective and neurorestorative effects...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779621/ https://www.ncbi.nlm.nih.gov/pubmed/33408606 http://dx.doi.org/10.3389/fnins.2020.575459 |
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author | Noonong, Kunwadee Sobhon, Prasert Sroyraya, Morakot Chaithirayanon, Kulathida |
author_facet | Noonong, Kunwadee Sobhon, Prasert Sroyraya, Morakot Chaithirayanon, Kulathida |
author_sort | Noonong, Kunwadee |
collection | PubMed |
description | Extracts from Holothuria scabra (HS) have been shown to possess anti-inflammation, anti-oxidant and anti-cancer activities. More recently, it was shown to have neuroprotective potential in Caenorhabditis elegans PD model. Here, we assessed whether HS has neuroprotective and neurorestorative effects on dopaminergic neurons in both mouse and cellular models of PD. We found that both pre-treatment and post-treatment with HS improved motor deficits in PD mouse model induced with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as determined by grid walk test. This was likely mediated by HS protective and restorative effects on maintaining the numbers of dopaminergic neurons and fibers in both substantia nigra pars compacta (SNpc) and striatum. In a cellular model of PD, HS significantly attenuated 1-methyl-4-phenylpyridinium (MPP(+))-induced apoptosis of DAergic-like neurons differentiated from SH-SY5Y cells by enhancing the expression of Bcl-2, suppressing the expression of cleaved Caspase 3 and preventing depolarization of mitochondrial membrane. In addition, HS could stimulate the expression of tyrosine hydroxylase (TH) and suppressed the formation of α-synuclein protein. Taken together, our in vivo and in vitro findings suggested that HS is an attractive candidate for the neuroprotection rather than neurorestoration in PD. |
format | Online Article Text |
id | pubmed-7779621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77796212021-01-05 Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease Noonong, Kunwadee Sobhon, Prasert Sroyraya, Morakot Chaithirayanon, Kulathida Front Neurosci Neuroscience Extracts from Holothuria scabra (HS) have been shown to possess anti-inflammation, anti-oxidant and anti-cancer activities. More recently, it was shown to have neuroprotective potential in Caenorhabditis elegans PD model. Here, we assessed whether HS has neuroprotective and neurorestorative effects on dopaminergic neurons in both mouse and cellular models of PD. We found that both pre-treatment and post-treatment with HS improved motor deficits in PD mouse model induced with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as determined by grid walk test. This was likely mediated by HS protective and restorative effects on maintaining the numbers of dopaminergic neurons and fibers in both substantia nigra pars compacta (SNpc) and striatum. In a cellular model of PD, HS significantly attenuated 1-methyl-4-phenylpyridinium (MPP(+))-induced apoptosis of DAergic-like neurons differentiated from SH-SY5Y cells by enhancing the expression of Bcl-2, suppressing the expression of cleaved Caspase 3 and preventing depolarization of mitochondrial membrane. In addition, HS could stimulate the expression of tyrosine hydroxylase (TH) and suppressed the formation of α-synuclein protein. Taken together, our in vivo and in vitro findings suggested that HS is an attractive candidate for the neuroprotection rather than neurorestoration in PD. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779621/ /pubmed/33408606 http://dx.doi.org/10.3389/fnins.2020.575459 Text en Copyright © 2020 Noonong, Sobhon, Sroyraya and Chaithirayanon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Noonong, Kunwadee Sobhon, Prasert Sroyraya, Morakot Chaithirayanon, Kulathida Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title | Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title_full | Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title_fullStr | Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title_full_unstemmed | Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title_short | Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP(+)-Induced Mouse and Cellular Models of Parkinson’s Disease |
title_sort | neuroprotective and neurorestorative effects of holothuria scabra extract in the mptp/mpp(+)-induced mouse and cellular models of parkinson’s disease |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779621/ https://www.ncbi.nlm.nih.gov/pubmed/33408606 http://dx.doi.org/10.3389/fnins.2020.575459 |
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