Cargando…

Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus

Differential levels of n-6 and n-3 essential polyunsaturated fatty acids (PUFAs) are incorporated into the hibernator’s diet in the fall season preceding prolonged, multi-days bouts of torpor, known as hibernation. Peroxisome proliferator-activated receptor (PPAR) transcriptional activators bind lip...

Descripción completa

Detalles Bibliográficos
Autores principales: Watts, Alexander J., Logan, Samantha M., Kübber-Heiss, Anna, Posautz, Annika, Stalder, Gabrielle, Painer, Johanna, Gasch, Kristina, Giroud, Sylvain, Storey, Kenneth B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779809/
https://www.ncbi.nlm.nih.gov/pubmed/33408645
http://dx.doi.org/10.3389/fphys.2020.615025
_version_ 1783631403918819328
author Watts, Alexander J.
Logan, Samantha M.
Kübber-Heiss, Anna
Posautz, Annika
Stalder, Gabrielle
Painer, Johanna
Gasch, Kristina
Giroud, Sylvain
Storey, Kenneth B.
author_facet Watts, Alexander J.
Logan, Samantha M.
Kübber-Heiss, Anna
Posautz, Annika
Stalder, Gabrielle
Painer, Johanna
Gasch, Kristina
Giroud, Sylvain
Storey, Kenneth B.
author_sort Watts, Alexander J.
collection PubMed
description Differential levels of n-6 and n-3 essential polyunsaturated fatty acids (PUFAs) are incorporated into the hibernator’s diet in the fall season preceding prolonged, multi-days bouts of torpor, known as hibernation. Peroxisome proliferator-activated receptor (PPAR) transcriptional activators bind lipids and regulate genes involved in fatty acid transport, beta-oxidation, ketogenesis, and insulin sensitivity; essential processes for survival during torpor. Thus, the DNA-binding activity of PPARα, PPARδ, PPARγ, as well as the levels of PPARγ coactivator 1α (PGC-1α) and L-fatty acid binding protein (L-FABP) were investigated in the hibernating garden dormouse (Eliomys quercinus). We found that dormice were hibernating in a similar way regardless of the n-6/n-3 PUFA diets fed to the animals during the fattening phase prior to hibernation. Further, metabolic rates and body mass loss during hibernation did not differ between dietary groups, despite marked differences in fatty acid profiles observed in white adipose tissue prior and at mid-hibernation. Overall, maintenance of PPAR DNA-binding activity was observed during torpor, and across three n-6/n-3 ratios, suggesting alternate mechanisms for the prioritization of lipid catabolism during torpor. Additionally, while no change was seen in L-FABP, significantly altered levels of PGC-1α were observed within the white adipose tissue and likely contributes to enhanced lipid metabolism when the diet favors n-6 PUFAs, i.e., high n-6/n-3 ratio, in both the torpid and euthermic state. Altogether, the maintenance of lipid metabolism during torpor makes it likely that consistent activity or levels of the investigated proteins are in aid of this metabolic profile.
format Online
Article
Text
id pubmed-7779809
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77798092021-01-05 Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus Watts, Alexander J. Logan, Samantha M. Kübber-Heiss, Anna Posautz, Annika Stalder, Gabrielle Painer, Johanna Gasch, Kristina Giroud, Sylvain Storey, Kenneth B. Front Physiol Physiology Differential levels of n-6 and n-3 essential polyunsaturated fatty acids (PUFAs) are incorporated into the hibernator’s diet in the fall season preceding prolonged, multi-days bouts of torpor, known as hibernation. Peroxisome proliferator-activated receptor (PPAR) transcriptional activators bind lipids and regulate genes involved in fatty acid transport, beta-oxidation, ketogenesis, and insulin sensitivity; essential processes for survival during torpor. Thus, the DNA-binding activity of PPARα, PPARδ, PPARγ, as well as the levels of PPARγ coactivator 1α (PGC-1α) and L-fatty acid binding protein (L-FABP) were investigated in the hibernating garden dormouse (Eliomys quercinus). We found that dormice were hibernating in a similar way regardless of the n-6/n-3 PUFA diets fed to the animals during the fattening phase prior to hibernation. Further, metabolic rates and body mass loss during hibernation did not differ between dietary groups, despite marked differences in fatty acid profiles observed in white adipose tissue prior and at mid-hibernation. Overall, maintenance of PPAR DNA-binding activity was observed during torpor, and across three n-6/n-3 ratios, suggesting alternate mechanisms for the prioritization of lipid catabolism during torpor. Additionally, while no change was seen in L-FABP, significantly altered levels of PGC-1α were observed within the white adipose tissue and likely contributes to enhanced lipid metabolism when the diet favors n-6 PUFAs, i.e., high n-6/n-3 ratio, in both the torpid and euthermic state. Altogether, the maintenance of lipid metabolism during torpor makes it likely that consistent activity or levels of the investigated proteins are in aid of this metabolic profile. Frontiers Media S.A. 2020-12-21 /pmc/articles/PMC7779809/ /pubmed/33408645 http://dx.doi.org/10.3389/fphys.2020.615025 Text en Copyright © 2020 Watts, Logan, Kübber-Heiss, Posautz, Stalder, Painer, Gasch, Giroud and Storey. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Watts, Alexander J.
Logan, Samantha M.
Kübber-Heiss, Anna
Posautz, Annika
Stalder, Gabrielle
Painer, Johanna
Gasch, Kristina
Giroud, Sylvain
Storey, Kenneth B.
Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title_full Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title_fullStr Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title_full_unstemmed Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title_short Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus
title_sort regulation of peroxisome proliferator-activated receptor pathway during torpor in the garden dormouse, eliomys quercinus
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779809/
https://www.ncbi.nlm.nih.gov/pubmed/33408645
http://dx.doi.org/10.3389/fphys.2020.615025
work_keys_str_mv AT wattsalexanderj regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT logansamantham regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT kubberheissanna regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT posautzannika regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT staldergabrielle regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT painerjohanna regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT gaschkristina regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT giroudsylvain regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus
AT storeykennethb regulationofperoxisomeproliferatoractivatedreceptorpathwayduringtorporinthegardendormouseeliomysquercinus