Protocol for generation of lung adenocarcinoma organoids from clinical samples

Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients’ responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma...

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Detalles Bibliográficos
Autores principales: Li, Zhichao, Yu, Lei, Chen, Dong, Meng, Zhaoyue, Chen, Wei, Huang, Weiren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779824/
https://www.ncbi.nlm.nih.gov/pubmed/33426535
http://dx.doi.org/10.1016/j.xpro.2020.100239
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author Li, Zhichao
Yu, Lei
Chen, Dong
Meng, Zhaoyue
Chen, Wei
Huang, Weiren
author_facet Li, Zhichao
Yu, Lei
Chen, Dong
Meng, Zhaoyue
Chen, Wei
Huang, Weiren
author_sort Li, Zhichao
collection PubMed
description Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients’ responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
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spelling pubmed-77798242021-01-08 Protocol for generation of lung adenocarcinoma organoids from clinical samples Li, Zhichao Yu, Lei Chen, Dong Meng, Zhaoyue Chen, Wei Huang, Weiren STAR Protoc Protocol Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients’ responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol. For complete details on the use and execution of this protocol, please refer to Li et al. (2020). Elsevier 2020-12-28 /pmc/articles/PMC7779824/ /pubmed/33426535 http://dx.doi.org/10.1016/j.xpro.2020.100239 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Zhichao
Yu, Lei
Chen, Dong
Meng, Zhaoyue
Chen, Wei
Huang, Weiren
Protocol for generation of lung adenocarcinoma organoids from clinical samples
title Protocol for generation of lung adenocarcinoma organoids from clinical samples
title_full Protocol for generation of lung adenocarcinoma organoids from clinical samples
title_fullStr Protocol for generation of lung adenocarcinoma organoids from clinical samples
title_full_unstemmed Protocol for generation of lung adenocarcinoma organoids from clinical samples
title_short Protocol for generation of lung adenocarcinoma organoids from clinical samples
title_sort protocol for generation of lung adenocarcinoma organoids from clinical samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779824/
https://www.ncbi.nlm.nih.gov/pubmed/33426535
http://dx.doi.org/10.1016/j.xpro.2020.100239
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