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Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome

Hepatocellular carcinoma (HCC) is one of the most frequently diagnosed types of cancer in the world. Post-translational modifications, such as phosphorylation, serve an essential role during cancer development. To identify aberrant phosphorylation in HCC, a multiplexed tandem mass tag approach combi...

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Autores principales: Liu, Yixian, Zhao, Qianwei, Xu, Fang, Wang, Kaijuan, Zhao, Ying, Chen, Huiping, He, Wei, Wang, Weidong, Zhang, Jianying, Zhang, Jintao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779902/
https://www.ncbi.nlm.nih.gov/pubmed/33408763
http://dx.doi.org/10.3892/ol.2020.12378
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author Liu, Yixian
Zhao, Qianwei
Xu, Fang
Wang, Kaijuan
Zhao, Ying
Chen, Huiping
He, Wei
Wang, Weidong
Zhang, Jianying
Zhang, Jintao
author_facet Liu, Yixian
Zhao, Qianwei
Xu, Fang
Wang, Kaijuan
Zhao, Ying
Chen, Huiping
He, Wei
Wang, Weidong
Zhang, Jianying
Zhang, Jintao
author_sort Liu, Yixian
collection PubMed
description Hepatocellular carcinoma (HCC) is one of the most frequently diagnosed types of cancer in the world. Post-translational modifications, such as phosphorylation, serve an essential role during cancer development. To identify aberrant phosphorylation in HCC, a multiplexed tandem mass tag approach combined with liquid chromatography tandem-mass spectrometry was used in the present study. The results are available via ProteomeXchange (identifier no. PXD013934). A total of 4,780 phosphorylated sites distributed on 2,209 proteins were identified and quantified, including 74 and 459 phosphorylated upregulated and downregulated proteins, respectively. Bioinformatic analysis revealed differences and similarities between HCC and normal tissues. Gene Ontology enrichment analysis provided information on biological processes, molecular functions, cellular components and sub-cellular localizations. Protein domains enrichment of differentially expressed proteins was analyzed using InterPro database. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed pathways that may potentially be involved in HCC. Integrative analysis of the functions, pathways, motifs of phosphorylated peptides, protein domains and protein interactions established a profile of the phosphoproteome of HCC, which may contribute to identify novel biomarkers for the diagnosis and prognosis of HCC, as well as novel therapeutic targets for HCC treatment.
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spelling pubmed-77799022021-01-05 Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome Liu, Yixian Zhao, Qianwei Xu, Fang Wang, Kaijuan Zhao, Ying Chen, Huiping He, Wei Wang, Weidong Zhang, Jianying Zhang, Jintao Oncol Lett Articles Hepatocellular carcinoma (HCC) is one of the most frequently diagnosed types of cancer in the world. Post-translational modifications, such as phosphorylation, serve an essential role during cancer development. To identify aberrant phosphorylation in HCC, a multiplexed tandem mass tag approach combined with liquid chromatography tandem-mass spectrometry was used in the present study. The results are available via ProteomeXchange (identifier no. PXD013934). A total of 4,780 phosphorylated sites distributed on 2,209 proteins were identified and quantified, including 74 and 459 phosphorylated upregulated and downregulated proteins, respectively. Bioinformatic analysis revealed differences and similarities between HCC and normal tissues. Gene Ontology enrichment analysis provided information on biological processes, molecular functions, cellular components and sub-cellular localizations. Protein domains enrichment of differentially expressed proteins was analyzed using InterPro database. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed pathways that may potentially be involved in HCC. Integrative analysis of the functions, pathways, motifs of phosphorylated peptides, protein domains and protein interactions established a profile of the phosphoproteome of HCC, which may contribute to identify novel biomarkers for the diagnosis and prognosis of HCC, as well as novel therapeutic targets for HCC treatment. D.A. Spandidos 2021-02 2020-12-15 /pmc/articles/PMC7779902/ /pubmed/33408763 http://dx.doi.org/10.3892/ol.2020.12378 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Yixian
Zhao, Qianwei
Xu, Fang
Wang, Kaijuan
Zhao, Ying
Chen, Huiping
He, Wei
Wang, Weidong
Zhang, Jianying
Zhang, Jintao
Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title_full Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title_fullStr Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title_full_unstemmed Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title_short Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
title_sort dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779902/
https://www.ncbi.nlm.nih.gov/pubmed/33408763
http://dx.doi.org/10.3892/ol.2020.12378
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