Cargando…

Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes

Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. gra...

Descripción completa

Detalles Bibliográficos
Autores principales: Aoun, Meriem, Kolmer, James A., Rouse, Matthew N., Chao, Shiaoman, Bulbula, Worku Denbel, Elias, Elias M., Acevedo, Maricelis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Phytopathological Society (APS) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779972/
https://www.ncbi.nlm.nih.gov/pubmed/28745102
http://dx.doi.org/10.1094/PHYTO-12-16-0444-R
_version_ 1783631423143411712
author Aoun, Meriem
Kolmer, James A.
Rouse, Matthew N.
Chao, Shiaoman
Bulbula, Worku Denbel
Elias, Elias M.
Acevedo, Maricelis
author_facet Aoun, Meriem
Kolmer, James A.
Rouse, Matthew N.
Chao, Shiaoman
Bulbula, Worku Denbel
Elias, Elias M.
Acevedo, Maricelis
author_sort Aoun, Meriem
collection PubMed
description Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single domi-nant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.
format Online
Article
Text
id pubmed-7779972
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The American Phytopathological Society (APS)
record_format MEDLINE/PubMed
spelling pubmed-77799722021-01-22 Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes Aoun, Meriem Kolmer, James A. Rouse, Matthew N. Chao, Shiaoman Bulbula, Worku Denbel Elias, Elias M. Acevedo, Maricelis Phytopathology Genetics and Resistance Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single domi-nant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum. The American Phytopathological Society (APS) 2017-09-29 2017 /pmc/articles/PMC7779972/ /pubmed/28745102 http://dx.doi.org/10.1094/PHYTO-12-16-0444-R Text en © 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the CC BY 4.0 International license.
spellingShingle Genetics and Resistance
Aoun, Meriem
Kolmer, James A.
Rouse, Matthew N.
Chao, Shiaoman
Bulbula, Worku Denbel
Elias, Elias M.
Acevedo, Maricelis
Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title_full Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title_fullStr Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title_full_unstemmed Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title_short Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
title_sort inheritance and bulked segregant analysis of leaf rust and stem rust resistance in durum wheat genotypes
topic Genetics and Resistance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779972/
https://www.ncbi.nlm.nih.gov/pubmed/28745102
http://dx.doi.org/10.1094/PHYTO-12-16-0444-R
work_keys_str_mv AT aounmeriem inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT kolmerjamesa inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT rousematthewn inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT chaoshiaoman inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT bulbulaworkudenbel inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT eliaseliasm inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes
AT acevedomaricelis inheritanceandbulkedsegregantanalysisofleafrustandstemrustresistanceindurumwheatgenotypes