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Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. gra...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Phytopathological Society (APS)
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779972/ https://www.ncbi.nlm.nih.gov/pubmed/28745102 http://dx.doi.org/10.1094/PHYTO-12-16-0444-R |
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author | Aoun, Meriem Kolmer, James A. Rouse, Matthew N. Chao, Shiaoman Bulbula, Worku Denbel Elias, Elias M. Acevedo, Maricelis |
author_facet | Aoun, Meriem Kolmer, James A. Rouse, Matthew N. Chao, Shiaoman Bulbula, Worku Denbel Elias, Elias M. Acevedo, Maricelis |
author_sort | Aoun, Meriem |
collection | PubMed |
description | Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single domi-nant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum. |
format | Online Article Text |
id | pubmed-7779972 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Phytopathological Society (APS) |
record_format | MEDLINE/PubMed |
spelling | pubmed-77799722021-01-22 Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes Aoun, Meriem Kolmer, James A. Rouse, Matthew N. Chao, Shiaoman Bulbula, Worku Denbel Elias, Elias M. Acevedo, Maricelis Phytopathology Genetics and Resistance Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single domi-nant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum. The American Phytopathological Society (APS) 2017-09-29 2017 /pmc/articles/PMC7779972/ /pubmed/28745102 http://dx.doi.org/10.1094/PHYTO-12-16-0444-R Text en © 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the CC BY 4.0 International license. |
spellingShingle | Genetics and Resistance Aoun, Meriem Kolmer, James A. Rouse, Matthew N. Chao, Shiaoman Bulbula, Worku Denbel Elias, Elias M. Acevedo, Maricelis Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes |
title | Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance
in Durum Wheat Genotypes |
title_full | Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance
in Durum Wheat Genotypes |
title_fullStr | Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance
in Durum Wheat Genotypes |
title_full_unstemmed | Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance
in Durum Wheat Genotypes |
title_short | Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance
in Durum Wheat Genotypes |
title_sort | inheritance and bulked segregant analysis of leaf rust and stem rust resistance
in durum wheat genotypes |
topic | Genetics and Resistance |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779972/ https://www.ncbi.nlm.nih.gov/pubmed/28745102 http://dx.doi.org/10.1094/PHYTO-12-16-0444-R |
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