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Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report
A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS patients to detect proteins in the culture supernatants from cells exposed to lavage another SARS patient. The most prominent protein in the supe...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780042/ https://www.ncbi.nlm.nih.gov/pubmed/12775768 http://dx.doi.org/10.1074/mcp.M300048-MCP200 |
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author | Krokhin, Oleg Li, Yan Andonov, Anton Feldmann, Heinz Flick, Ramon Jones, Steven Stroeher, Ute Bastien, Nathalie Dasuri, Kumar V.N. Cheng, Keding Simonsen, J. Neil Perreault, Hélène Wilkins, John Ens, Werner Plummer, Frank Standing, Kenneth G. |
author_facet | Krokhin, Oleg Li, Yan Andonov, Anton Feldmann, Heinz Flick, Ramon Jones, Steven Stroeher, Ute Bastien, Nathalie Dasuri, Kumar V.N. Cheng, Keding Simonsen, J. Neil Perreault, Hélène Wilkins, John Ens, Werner Plummer, Frank Standing, Kenneth G. |
author_sort | Krokhin, Oleg |
collection | PubMed |
description | A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS patients to detect proteins in the culture supernatants from cells exposed to lavage another SARS patient. The most prominent protein in the supernatant was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a ∼46-kDa species. This was found to be a novel nucleocapsid protein that matched almost exactly one predicted by an open reading frame in the recently published nucleotide sequence of the same virus isolate (>96% coverage). A second viral protein corresponding to the predicted ∼139-kDa spike glycoprotein has also been examined by MALDI-TOF MS (42% coverage). After peptide N-glycosidase F digestion, 12 glycosylation sites in this protein were confirmed. The sugars attached to four of the sites were also identified. These results suggest that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics, and that the spike glycoprotein may present a particularly attractive target for prophylactic intervention in combating SARS. |
format | Online Article Text |
id | pubmed-7780042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77800422021-01-04 Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report Krokhin, Oleg Li, Yan Andonov, Anton Feldmann, Heinz Flick, Ramon Jones, Steven Stroeher, Ute Bastien, Nathalie Dasuri, Kumar V.N. Cheng, Keding Simonsen, J. Neil Perreault, Hélène Wilkins, John Ens, Werner Plummer, Frank Standing, Kenneth G. Mol Cell Proteomics Research A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS patients to detect proteins in the culture supernatants from cells exposed to lavage another SARS patient. The most prominent protein in the supernatant was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a ∼46-kDa species. This was found to be a novel nucleocapsid protein that matched almost exactly one predicted by an open reading frame in the recently published nucleotide sequence of the same virus isolate (>96% coverage). A second viral protein corresponding to the predicted ∼139-kDa spike glycoprotein has also been examined by MALDI-TOF MS (42% coverage). After peptide N-glycosidase F digestion, 12 glycosylation sites in this protein were confirmed. The sugars attached to four of the sites were also identified. These results suggest that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics, and that the spike glycoprotein may present a particularly attractive target for prophylactic intervention in combating SARS. ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. 2003-05-01 2021-01-04 /pmc/articles/PMC7780042/ /pubmed/12775768 http://dx.doi.org/10.1074/mcp.M300048-MCP200 Text en © 2003 © 2003 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Research Krokhin, Oleg Li, Yan Andonov, Anton Feldmann, Heinz Flick, Ramon Jones, Steven Stroeher, Ute Bastien, Nathalie Dasuri, Kumar V.N. Cheng, Keding Simonsen, J. Neil Perreault, Hélène Wilkins, John Ens, Werner Plummer, Frank Standing, Kenneth G. Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title | Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title_full | Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title_fullStr | Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title_full_unstemmed | Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title_short | Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report |
title_sort | mass spectrometric characterization of proteins from the sars virus: a preliminary report |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780042/ https://www.ncbi.nlm.nih.gov/pubmed/12775768 http://dx.doi.org/10.1074/mcp.M300048-MCP200 |
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