A practical microfluidic pump enabled by acoustofluidics and 3D printing

Simple and low-cost solutions are becoming extremely important for the evolving necessities of biomedical applications. Even though, on-chip sample processing and analysis has been rapidly developing for a wide range of screening and diagnostic protocols, efficient and reliable fluid manipulation in microfluidic platforms still require further developments to be considered portable and accessible for low-resource settings. In this work, we present an extremely simple microfluidic pumping device based on three-dimensional (3D) printing and acoustofluidics. The fabrication of the device only requires 3D-printed adaptors, rectangular glass capillaries, epoxy and a piezoelectric transducer. The pumping mechanism relies on the flexibility and complexity of the acoustic streaming patterns generated inside the capillary. Characterization of the device yields controllable and continuous flow rates suitable for on-chip sample processing and analysis. Overall, a maximum flow rate of ~ 12 μL/min and the control of pumping direction by frequency tuning is achieved. With its versatility and simplicity, this microfluidic pumping device offers a promising solution for portable, affordable and reliable fluid manipulation for on-chip applications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10404-020-02411-w.