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Ultrasonic techniques to obtain dental pulp from impacted third molars

BACKGROUND: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity. Extracted teeth, usually discarded, have been revalued as bio...

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Autores principales: Becchio, Jorge G., Rosende, Roque O., Monzón, Jorge E., Fernández, Darío, Said-Rücker, Patricia B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781209/
https://www.ncbi.nlm.nih.gov/pubmed/33425224
http://dx.doi.org/10.4317/jced.56658
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author Becchio, Jorge G.
Rosende, Roque O.
Monzón, Jorge E.
Fernández, Darío
Said-Rücker, Patricia B.
author_facet Becchio, Jorge G.
Rosende, Roque O.
Monzón, Jorge E.
Fernández, Darío
Said-Rücker, Patricia B.
author_sort Becchio, Jorge G.
collection PubMed
description BACKGROUND: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity. Extracted teeth, usually discarded, have been revalued as biological material, providing living tissues with possible applications in regenerative dentistry. The aim was to compare cross-section methods of upper included third molars by ultrasonic piezoelectric technique to obtain dental pulp, with diamond-coated tip (DT) against titanium nitride-coated tip (TN), according to the pulp tissue cell viability and the section surface characteristics. MATERIAL AND METHODS: Patients attending dental consultation were evaluated. Upper third molars (n= 24) were avulsed from 15 patients with exodontia indication, age 18-26 years old, who agreed to participate of the study. Third molars were cross-sectioned at amelocemental junction level with piezoelectric device using DT or TN inserts. Pulps were mechanic and enzymatically treated, and tissue viability determined by Trypan Blue test. Sectioned teeth were visualized using Scanning Electron Microscope (SEM). Ethical principles of biomedical research were respected; all patients gave their informed consent. RESULTS: Viability of pulp tissue was 84.71% not associated to sex (p= 0.141) nor to teeth position, upper right third molar or upper left third molar (p= 0.580). According to the insert used, pulp tissue viability was 85.21% with TN, similar to 84.00% with DT (p= 0.611). By SEM, cut performed by TN insert showed smooth and uniform surfaces, while DT insert surfaces were irregular, porous, with fissures. CONCLUSIONS: Piezosurgery applied to cross-section upper third molars with both types of inserts showed differences in the cut surfaces but similar effectiveness regarding preservation of pulp tissue with high viability, thus, they could be allocated for further cellular developments. Key words:Impacted teeth, third molars, piezosurgery, regenerative dentistry.
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spelling pubmed-77812092021-01-07 Ultrasonic techniques to obtain dental pulp from impacted third molars Becchio, Jorge G. Rosende, Roque O. Monzón, Jorge E. Fernández, Darío Said-Rücker, Patricia B. J Clin Exp Dent Research BACKGROUND: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity. Extracted teeth, usually discarded, have been revalued as biological material, providing living tissues with possible applications in regenerative dentistry. The aim was to compare cross-section methods of upper included third molars by ultrasonic piezoelectric technique to obtain dental pulp, with diamond-coated tip (DT) against titanium nitride-coated tip (TN), according to the pulp tissue cell viability and the section surface characteristics. MATERIAL AND METHODS: Patients attending dental consultation were evaluated. Upper third molars (n= 24) were avulsed from 15 patients with exodontia indication, age 18-26 years old, who agreed to participate of the study. Third molars were cross-sectioned at amelocemental junction level with piezoelectric device using DT or TN inserts. Pulps were mechanic and enzymatically treated, and tissue viability determined by Trypan Blue test. Sectioned teeth were visualized using Scanning Electron Microscope (SEM). Ethical principles of biomedical research were respected; all patients gave their informed consent. RESULTS: Viability of pulp tissue was 84.71% not associated to sex (p= 0.141) nor to teeth position, upper right third molar or upper left third molar (p= 0.580). According to the insert used, pulp tissue viability was 85.21% with TN, similar to 84.00% with DT (p= 0.611). By SEM, cut performed by TN insert showed smooth and uniform surfaces, while DT insert surfaces were irregular, porous, with fissures. CONCLUSIONS: Piezosurgery applied to cross-section upper third molars with both types of inserts showed differences in the cut surfaces but similar effectiveness regarding preservation of pulp tissue with high viability, thus, they could be allocated for further cellular developments. Key words:Impacted teeth, third molars, piezosurgery, regenerative dentistry. Medicina Oral S.L. 2021-01-01 /pmc/articles/PMC7781209/ /pubmed/33425224 http://dx.doi.org/10.4317/jced.56658 Text en Copyright: © 2021 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Becchio, Jorge G.
Rosende, Roque O.
Monzón, Jorge E.
Fernández, Darío
Said-Rücker, Patricia B.
Ultrasonic techniques to obtain dental pulp from impacted third molars
title Ultrasonic techniques to obtain dental pulp from impacted third molars
title_full Ultrasonic techniques to obtain dental pulp from impacted third molars
title_fullStr Ultrasonic techniques to obtain dental pulp from impacted third molars
title_full_unstemmed Ultrasonic techniques to obtain dental pulp from impacted third molars
title_short Ultrasonic techniques to obtain dental pulp from impacted third molars
title_sort ultrasonic techniques to obtain dental pulp from impacted third molars
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781209/
https://www.ncbi.nlm.nih.gov/pubmed/33425224
http://dx.doi.org/10.4317/jced.56658
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