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Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments
Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the s...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781620/ https://www.ncbi.nlm.nih.gov/pubmed/33382949 http://dx.doi.org/10.1080/19420862.2020.1864084 |