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Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation

This study aimed at evaluating the cytoprotective activity of jujube water extract (JWE) against alcohol-induced oxidative stress via the activation of the Nrf2 pathway in HepG2 cells. JWE had various phenolic compounds, and the vanillic acid content was the highest in the extract. To determine the...

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Autores principales: Hong, Sungwha, Kim, Younghwa, Sung, Jeehye, Lee, Hana, Heo, Huijin, Jeong, Heon Sang, Lee, Junsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781690/
https://www.ncbi.nlm.nih.gov/pubmed/33424992
http://dx.doi.org/10.1155/2020/6684331
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author Hong, Sungwha
Kim, Younghwa
Sung, Jeehye
Lee, Hana
Heo, Huijin
Jeong, Heon Sang
Lee, Junsoo
author_facet Hong, Sungwha
Kim, Younghwa
Sung, Jeehye
Lee, Hana
Heo, Huijin
Jeong, Heon Sang
Lee, Junsoo
author_sort Hong, Sungwha
collection PubMed
description This study aimed at evaluating the cytoprotective activity of jujube water extract (JWE) against alcohol-induced oxidative stress via the activation of the Nrf2 pathway in HepG2 cells. JWE had various phenolic compounds, and the vanillic acid content was the highest in the extract. To determine the cytoprotective effect of JWE against alcohol-induced damage, hepatocytes were treated with JWE and 3% ethanol. JWE (100 μg/mL) markedly increased cell viability by approximately 100% in a dose-dependent manner. Moreover, JWE attenuated the production of malondialdehyde, reactive oxygen species, aspartate, and alanine aminotransferase and the depletion of glutathione. Moreover, JWE enhanced the expression of antioxidant defense enzymes including heme oxygenase-1, NADPH quinone oxidoreductase 1, and γ-glutamate-cysteine ligase catalytic against alcohol-induced oxidative damage in hepatocytes via the activation of Nrf2. Taken together, JWE possesses the protective effect against alcohol-induced oxidative injury in hepatocytes through the upregulation of the Nrf2 signaling pathway. Therefore, jujube fruit might have the potential to improve alcohol-related liver problems.
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spelling pubmed-77816902021-01-08 Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation Hong, Sungwha Kim, Younghwa Sung, Jeehye Lee, Hana Heo, Huijin Jeong, Heon Sang Lee, Junsoo Evid Based Complement Alternat Med Research Article This study aimed at evaluating the cytoprotective activity of jujube water extract (JWE) against alcohol-induced oxidative stress via the activation of the Nrf2 pathway in HepG2 cells. JWE had various phenolic compounds, and the vanillic acid content was the highest in the extract. To determine the cytoprotective effect of JWE against alcohol-induced damage, hepatocytes were treated with JWE and 3% ethanol. JWE (100 μg/mL) markedly increased cell viability by approximately 100% in a dose-dependent manner. Moreover, JWE attenuated the production of malondialdehyde, reactive oxygen species, aspartate, and alanine aminotransferase and the depletion of glutathione. Moreover, JWE enhanced the expression of antioxidant defense enzymes including heme oxygenase-1, NADPH quinone oxidoreductase 1, and γ-glutamate-cysteine ligase catalytic against alcohol-induced oxidative damage in hepatocytes via the activation of Nrf2. Taken together, JWE possesses the protective effect against alcohol-induced oxidative injury in hepatocytes through the upregulation of the Nrf2 signaling pathway. Therefore, jujube fruit might have the potential to improve alcohol-related liver problems. Hindawi 2020-12-24 /pmc/articles/PMC7781690/ /pubmed/33424992 http://dx.doi.org/10.1155/2020/6684331 Text en Copyright © 2020 Sungwha Hong et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hong, Sungwha
Kim, Younghwa
Sung, Jeehye
Lee, Hana
Heo, Huijin
Jeong, Heon Sang
Lee, Junsoo
Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title_full Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title_fullStr Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title_full_unstemmed Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title_short Jujube (Ziziphus jujuba Mill.) Protects Hepatocytes against Alcohol-Induced Damage through Nrf2 Activation
title_sort jujube (ziziphus jujuba mill.) protects hepatocytes against alcohol-induced damage through nrf2 activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781690/
https://www.ncbi.nlm.nih.gov/pubmed/33424992
http://dx.doi.org/10.1155/2020/6684331
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