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ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia

BACKGROUND: Hyperoxia treats a subset of critical neonatal illnesses but induces intestinal damage in neonatal pups. In this process, the intestinal flora and mucosal epithelium might be altered by hyperoxia. So the changes of the intestinal flora and mucosal epithelium were studied. METHODS: Neonat...

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Autores principales: Liu, D. Y., Lou, W. J., Zhang, D. Y., Sun, S. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781695/
https://www.ncbi.nlm.nih.gov/pubmed/33426071
http://dx.doi.org/10.1155/2020/8819195
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author Liu, D. Y.
Lou, W. J.
Zhang, D. Y.
Sun, S. Y.
author_facet Liu, D. Y.
Lou, W. J.
Zhang, D. Y.
Sun, S. Y.
author_sort Liu, D. Y.
collection PubMed
description BACKGROUND: Hyperoxia treats a subset of critical neonatal illnesses but induces intestinal damage in neonatal pups. In this process, the intestinal flora and mucosal epithelium might be altered by hyperoxia. So the changes of the intestinal flora and mucosal epithelium were studied. METHODS: Neonatal rats were randomized into the model group that was exposed to hyperoxia and the control group that was maintained under normoxic conditions; then, intestinal lavage fluid and intestinal tissues were harvested. ELISA was used to detect D-lactic acid (D-LA), endotoxin (ET), diamine oxidase (DAO), intestinal fatty acid binding protein (i-FABP), liver-type fatty acid binding protein (L-FABP) and cytokines in the intestinal lavage of neonatal rats during hyperoxia. The intestinal zonula occluden-1 (ZO-1), occlusion protein (Occludin), and closure protein-4 (Claudin-4) of neonatal pups were detected by immunohistochemistry, western blotting, and real-time Polymerase chain reaction (RT-PCR) during hyperoxia. NCM460 cell survival rates were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) during hyperoxia and administration of N-acetyl-L-cysteine (NAC). The expression levels of ZO-1, Occludin, and Claudin-4 in NCM460 cells were detected by immunohistochemistry, western blotting, and RT-PCR during hyperoxia and NAC. RESULTS: D-LA, ET, L-FABP, i-FABP, DAO, TNF-α, IL-10, and IFN-γ were significantly increased by hyperoxia, while ZO-1, Occludin, and Claudin-4 were clearly decreased in the hyperoxia group compared with the control group. NAC promoted cell survival, which was inhibited by hyperoxia. The cellular expression levels of ZO-1, Occludin, and Claudin-4, which were lowered by hyperoxia, were increased by NAC. CONCLUSION: Hyperoxia causes injury of the intestinal mucosa, and ROS plays a role in this intestinal damage during hyperoxia.
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spelling pubmed-77816952021-01-08 ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia Liu, D. Y. Lou, W. J. Zhang, D. Y. Sun, S. Y. Biomed Res Int Research Article BACKGROUND: Hyperoxia treats a subset of critical neonatal illnesses but induces intestinal damage in neonatal pups. In this process, the intestinal flora and mucosal epithelium might be altered by hyperoxia. So the changes of the intestinal flora and mucosal epithelium were studied. METHODS: Neonatal rats were randomized into the model group that was exposed to hyperoxia and the control group that was maintained under normoxic conditions; then, intestinal lavage fluid and intestinal tissues were harvested. ELISA was used to detect D-lactic acid (D-LA), endotoxin (ET), diamine oxidase (DAO), intestinal fatty acid binding protein (i-FABP), liver-type fatty acid binding protein (L-FABP) and cytokines in the intestinal lavage of neonatal rats during hyperoxia. The intestinal zonula occluden-1 (ZO-1), occlusion protein (Occludin), and closure protein-4 (Claudin-4) of neonatal pups were detected by immunohistochemistry, western blotting, and real-time Polymerase chain reaction (RT-PCR) during hyperoxia. NCM460 cell survival rates were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) during hyperoxia and administration of N-acetyl-L-cysteine (NAC). The expression levels of ZO-1, Occludin, and Claudin-4 in NCM460 cells were detected by immunohistochemistry, western blotting, and RT-PCR during hyperoxia and NAC. RESULTS: D-LA, ET, L-FABP, i-FABP, DAO, TNF-α, IL-10, and IFN-γ were significantly increased by hyperoxia, while ZO-1, Occludin, and Claudin-4 were clearly decreased in the hyperoxia group compared with the control group. NAC promoted cell survival, which was inhibited by hyperoxia. The cellular expression levels of ZO-1, Occludin, and Claudin-4, which were lowered by hyperoxia, were increased by NAC. CONCLUSION: Hyperoxia causes injury of the intestinal mucosa, and ROS plays a role in this intestinal damage during hyperoxia. Hindawi 2020-12-26 /pmc/articles/PMC7781695/ /pubmed/33426071 http://dx.doi.org/10.1155/2020/8819195 Text en Copyright © 2020 D. Y. Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, D. Y.
Lou, W. J.
Zhang, D. Y.
Sun, S. Y.
ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title_full ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title_fullStr ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title_full_unstemmed ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title_short ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia
title_sort ros plays a role in the neonatal rat intestinal barrier damages induced by hyperoxia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781695/
https://www.ncbi.nlm.nih.gov/pubmed/33426071
http://dx.doi.org/10.1155/2020/8819195
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