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Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa
Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ub...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782355/ https://www.ncbi.nlm.nih.gov/pubmed/33414776 http://dx.doi.org/10.3389/fmicb.2020.605229 |
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author | Zheng, Xin Gao, Shunxiang Wu, Jihong Hu, Xiaobo |
author_facet | Zheng, Xin Gao, Shunxiang Wu, Jihong Hu, Xiaobo |
author_sort | Zheng, Xin |
collection | PubMed |
description | Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined. |
format | Online Article Text |
id | pubmed-7782355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77823552021-01-06 Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa Zheng, Xin Gao, Shunxiang Wu, Jihong Hu, Xiaobo Front Microbiol Microbiology Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined. Frontiers Media S.A. 2020-12-22 /pmc/articles/PMC7782355/ /pubmed/33414776 http://dx.doi.org/10.3389/fmicb.2020.605229 Text en Copyright © 2020 Zheng, Gao, Wu and Hu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zheng, Xin Gao, Shunxiang Wu, Jihong Hu, Xiaobo Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title | Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title_full | Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title_fullStr | Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title_full_unstemmed | Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title_short | Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa |
title_sort | recent advances in aptamer-based biosensors for detection of pseudomonas aeruginosa |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782355/ https://www.ncbi.nlm.nih.gov/pubmed/33414776 http://dx.doi.org/10.3389/fmicb.2020.605229 |
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