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Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure
The aim of this prospective study was to evaluate outcome benefits expected in repeated implantation failure (RIF) patients (n = 217) after customized embryo transfer based upon identification of the receptivity window by transcriptomic approach using the Win-Test. In this test, the expression of 11...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782404/ https://www.ncbi.nlm.nih.gov/pubmed/32725589 http://dx.doi.org/10.1007/s43032-020-00252-0 |
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author | Haouzi, Delphine Entezami, Frida Torre, Antoine Innocenti, Charlène Antoine, Yannick Mauries, Charlotte Vincens, Claire Bringer-Deutsch, Sophie Gala, Anna Ferrieres-HOA, Alice Ohl, Jeanine Gonzalez Marti, Beatriz Brouillet, Sophie Hamamah, Samir |
author_facet | Haouzi, Delphine Entezami, Frida Torre, Antoine Innocenti, Charlène Antoine, Yannick Mauries, Charlotte Vincens, Claire Bringer-Deutsch, Sophie Gala, Anna Ferrieres-HOA, Alice Ohl, Jeanine Gonzalez Marti, Beatriz Brouillet, Sophie Hamamah, Samir |
author_sort | Haouzi, Delphine |
collection | PubMed |
description | The aim of this prospective study was to evaluate outcome benefits expected in repeated implantation failure (RIF) patients (n = 217) after customized embryo transfer based upon identification of the receptivity window by transcriptomic approach using the Win-Test. In this test, the expression of 11 endometrial genes known to be predictive of endometrial receptivity is assessed by RT-PCR in biopsies collected during the implantation window (6–9 days after the spontaneous luteinizing hormone surge during natural cycles, 5–9 days after progesterone administration during hormone replacement therapy cycles). Then, patients underwent either customized embryo transfer (cET, n = 157 patients) according to the Win-Test results or embryo transfer according to the classical procedure (control group, n = 60). Pregnancy and live birth rates were compared in the two groups. The Win-Test showed that in 78.5% of women, the receptivity window lasted less than 48 h, although it could be shorter (< 24 h, 9.5%) or longer (> 48 h, 12%). This highlighted that only in 20% of patients with RIF the endometrium would have been receptive if the classical embryo transfer protocol was followed. In the other 80% of patients, the receptivity window was delayed by 1–3 days relative to the classical timing. This suggests that implantation failure could be linked to inadequate timing of embryo transfer. In agreement, both implantation (22.7% vs. 7.2%) and live birth rates per patient (31.8% vs. 8.3%) were significantly higher in the cET group than in the control group. cET on the basis of the Win-Test results could be proposed to improve pregnancy and live birth rates. ClinicalTrials.gov ID: NCT04192396; December 5, 2019, retrospectively registered. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s43032-020-00252-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7782404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-77824042021-01-11 Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure Haouzi, Delphine Entezami, Frida Torre, Antoine Innocenti, Charlène Antoine, Yannick Mauries, Charlotte Vincens, Claire Bringer-Deutsch, Sophie Gala, Anna Ferrieres-HOA, Alice Ohl, Jeanine Gonzalez Marti, Beatriz Brouillet, Sophie Hamamah, Samir Reprod Sci Original Article The aim of this prospective study was to evaluate outcome benefits expected in repeated implantation failure (RIF) patients (n = 217) after customized embryo transfer based upon identification of the receptivity window by transcriptomic approach using the Win-Test. In this test, the expression of 11 endometrial genes known to be predictive of endometrial receptivity is assessed by RT-PCR in biopsies collected during the implantation window (6–9 days after the spontaneous luteinizing hormone surge during natural cycles, 5–9 days after progesterone administration during hormone replacement therapy cycles). Then, patients underwent either customized embryo transfer (cET, n = 157 patients) according to the Win-Test results or embryo transfer according to the classical procedure (control group, n = 60). Pregnancy and live birth rates were compared in the two groups. The Win-Test showed that in 78.5% of women, the receptivity window lasted less than 48 h, although it could be shorter (< 24 h, 9.5%) or longer (> 48 h, 12%). This highlighted that only in 20% of patients with RIF the endometrium would have been receptive if the classical embryo transfer protocol was followed. In the other 80% of patients, the receptivity window was delayed by 1–3 days relative to the classical timing. This suggests that implantation failure could be linked to inadequate timing of embryo transfer. In agreement, both implantation (22.7% vs. 7.2%) and live birth rates per patient (31.8% vs. 8.3%) were significantly higher in the cET group than in the control group. cET on the basis of the Win-Test results could be proposed to improve pregnancy and live birth rates. ClinicalTrials.gov ID: NCT04192396; December 5, 2019, retrospectively registered. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s43032-020-00252-0) contains supplementary material, which is available to authorized users. Springer International Publishing 2020-07-28 /pmc/articles/PMC7782404/ /pubmed/32725589 http://dx.doi.org/10.1007/s43032-020-00252-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Article Haouzi, Delphine Entezami, Frida Torre, Antoine Innocenti, Charlène Antoine, Yannick Mauries, Charlotte Vincens, Claire Bringer-Deutsch, Sophie Gala, Anna Ferrieres-HOA, Alice Ohl, Jeanine Gonzalez Marti, Beatriz Brouillet, Sophie Hamamah, Samir Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title | Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title_full | Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title_fullStr | Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title_full_unstemmed | Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title_short | Customized Frozen Embryo Transfer after Identification of the Receptivity Window with a Transcriptomic Approach Improves the Implantation and Live Birth Rates in Patients with Repeated Implantation Failure |
title_sort | customized frozen embryo transfer after identification of the receptivity window with a transcriptomic approach improves the implantation and live birth rates in patients with repeated implantation failure |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782404/ https://www.ncbi.nlm.nih.gov/pubmed/32725589 http://dx.doi.org/10.1007/s43032-020-00252-0 |
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