Cargando…

Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen

During pollen maturation, various organelles change their distribution and function during development as male gametophytes. We analyzed the behavior of lipid bodies and vacuoles involved in lipophagy in Arabidopsis pollen using serial section SEM and conventional TEM. At the bicellular pollen stage...

Descripción completa

Detalles Bibliográficos
Autores principales: Akita, Kae, Takagi, Tomoko, Kobayashi, Keiko, Kuchitsu, Kazuyuki, Kuroiwa, Tsuneyoshi, Nagata, Noriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782417/
https://www.ncbi.nlm.nih.gov/pubmed/32968871
http://dx.doi.org/10.1007/s00709-020-01557-2
_version_ 1783631897725763584
author Akita, Kae
Takagi, Tomoko
Kobayashi, Keiko
Kuchitsu, Kazuyuki
Kuroiwa, Tsuneyoshi
Nagata, Noriko
author_facet Akita, Kae
Takagi, Tomoko
Kobayashi, Keiko
Kuchitsu, Kazuyuki
Kuroiwa, Tsuneyoshi
Nagata, Noriko
author_sort Akita, Kae
collection PubMed
description During pollen maturation, various organelles change their distribution and function during development as male gametophytes. We analyzed the behavior of lipid bodies and vacuoles involved in lipophagy in Arabidopsis pollen using serial section SEM and conventional TEM. At the bicellular pollen stage, lipid bodies in the vegetative cells lined up at the surface of the generative cell. Vacuoles then tightly attached, drew in, and degraded the lipid bodies and eventually occupied the space of the lipid bodies. Degradation of lipid began before transfer of the entire contents of the lipid body. At the tricellular stage, vacuoles instead of lipid bodies surrounded the sperm cells. The degradation of lipid bodies is morphologically considered microautophagy. The atg2-1 Arabidopsis mutant is deficient in one autophagy-related gene (ATG). In this mutant, the assembly of vacuoles around sperm cells was sparser than that in wild-type pollen. The deficiency of ATG2 likely prevents or slows lipid degradation, although it does not prevent contact between organelles. These results demonstrate the involvement of microlipophagy in the pollen development of Arabidopsis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00709-020-01557-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-7782417
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-77824172021-01-11 Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen Akita, Kae Takagi, Tomoko Kobayashi, Keiko Kuchitsu, Kazuyuki Kuroiwa, Tsuneyoshi Nagata, Noriko Protoplasma Original Article During pollen maturation, various organelles change their distribution and function during development as male gametophytes. We analyzed the behavior of lipid bodies and vacuoles involved in lipophagy in Arabidopsis pollen using serial section SEM and conventional TEM. At the bicellular pollen stage, lipid bodies in the vegetative cells lined up at the surface of the generative cell. Vacuoles then tightly attached, drew in, and degraded the lipid bodies and eventually occupied the space of the lipid bodies. Degradation of lipid began before transfer of the entire contents of the lipid body. At the tricellular stage, vacuoles instead of lipid bodies surrounded the sperm cells. The degradation of lipid bodies is morphologically considered microautophagy. The atg2-1 Arabidopsis mutant is deficient in one autophagy-related gene (ATG). In this mutant, the assembly of vacuoles around sperm cells was sparser than that in wild-type pollen. The deficiency of ATG2 likely prevents or slows lipid degradation, although it does not prevent contact between organelles. These results demonstrate the involvement of microlipophagy in the pollen development of Arabidopsis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00709-020-01557-2) contains supplementary material, which is available to authorized users. Springer Vienna 2020-09-23 2021 /pmc/articles/PMC7782417/ /pubmed/32968871 http://dx.doi.org/10.1007/s00709-020-01557-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Akita, Kae
Takagi, Tomoko
Kobayashi, Keiko
Kuchitsu, Kazuyuki
Kuroiwa, Tsuneyoshi
Nagata, Noriko
Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title_full Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title_fullStr Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title_full_unstemmed Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title_short Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen
title_sort ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in arabidopsis pollen
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782417/
https://www.ncbi.nlm.nih.gov/pubmed/32968871
http://dx.doi.org/10.1007/s00709-020-01557-2
work_keys_str_mv AT akitakae ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen
AT takagitomoko ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen
AT kobayashikeiko ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen
AT kuchitsukazuyuki ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen
AT kuroiwatsuneyoshi ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen
AT nagatanoriko ultrastructuralcharacterizationofmicrolipophagyinducedbytheinteractionofvacuolesandlipidbodiesaroundgenerativeandspermcellsinarabidopsispollen