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Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China

Salmonella is an important food pathogen that can cause severe gastroenteritis with more than 600,000 deaths globally every year. Colistin (COL), a last-resort antibiotic, is ineffective in bacteria that carry a functional mcr-1 gene, which is often spread by conjugative plasmids. Our work aimed to...

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Autores principales: Fan, Jianzhong, Zhang, Linghong, He, Jintao, Zhao, Maoying, Loh, Belinda, Leptihn, Sebastian, Yu, Yunsong, Hua, Xiaoting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782425/
https://www.ncbi.nlm.nih.gov/pubmed/33414775
http://dx.doi.org/10.3389/fmicb.2020.604710
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author Fan, Jianzhong
Zhang, Linghong
He, Jintao
Zhao, Maoying
Loh, Belinda
Leptihn, Sebastian
Yu, Yunsong
Hua, Xiaoting
author_facet Fan, Jianzhong
Zhang, Linghong
He, Jintao
Zhao, Maoying
Loh, Belinda
Leptihn, Sebastian
Yu, Yunsong
Hua, Xiaoting
author_sort Fan, Jianzhong
collection PubMed
description Salmonella is an important food pathogen that can cause severe gastroenteritis with more than 600,000 deaths globally every year. Colistin (COL), a last-resort antibiotic, is ineffective in bacteria that carry a functional mcr-1 gene, which is often spread by conjugative plasmids. Our work aimed to understand the prevalence of the mcr-1 gene in clinical isolates of Salmonella, as the frequency of occurrence of the mcr-1 gene is increasing globally. Therefore, we analyzed 689 clinical strains, that were isolated between 2009 and late 2018. The mcr-1 gene was found in six strains, which we analyzed in detail by whole genome sequencing and antibiotic susceptibility testing, while we also provide the clinical information on the patients suffering from an infection. The genomic analysis revealed that five strains had plasmid-encoded mcr-1 gene located in four IncHI2 plasmids and one IncI2 plasmid, while one strain had the chromosomal mcr-1 gene originated from plasmid. Surprisingly, in two strains the mcr-1 genes were inactive due to disruption by insertion sequences (ISs): ISApl1 and ISVsa5. A detailed analysis of the plasmids revealed a multitude of ISs, most commonly IS26. The IS contained genes that meditate broad resistance toward most antibiotics underlining their importance of the mobile elements, also with respect to the spread of the mcr-1 gene. Our study revealed potential reservoirs for the transmission of COL resistance and offers insights into the evolution of the mcr-1 gene in Salmonella.
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spelling pubmed-77824252021-01-06 Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China Fan, Jianzhong Zhang, Linghong He, Jintao Zhao, Maoying Loh, Belinda Leptihn, Sebastian Yu, Yunsong Hua, Xiaoting Front Microbiol Microbiology Salmonella is an important food pathogen that can cause severe gastroenteritis with more than 600,000 deaths globally every year. Colistin (COL), a last-resort antibiotic, is ineffective in bacteria that carry a functional mcr-1 gene, which is often spread by conjugative plasmids. Our work aimed to understand the prevalence of the mcr-1 gene in clinical isolates of Salmonella, as the frequency of occurrence of the mcr-1 gene is increasing globally. Therefore, we analyzed 689 clinical strains, that were isolated between 2009 and late 2018. The mcr-1 gene was found in six strains, which we analyzed in detail by whole genome sequencing and antibiotic susceptibility testing, while we also provide the clinical information on the patients suffering from an infection. The genomic analysis revealed that five strains had plasmid-encoded mcr-1 gene located in four IncHI2 plasmids and one IncI2 plasmid, while one strain had the chromosomal mcr-1 gene originated from plasmid. Surprisingly, in two strains the mcr-1 genes were inactive due to disruption by insertion sequences (ISs): ISApl1 and ISVsa5. A detailed analysis of the plasmids revealed a multitude of ISs, most commonly IS26. The IS contained genes that meditate broad resistance toward most antibiotics underlining their importance of the mobile elements, also with respect to the spread of the mcr-1 gene. Our study revealed potential reservoirs for the transmission of COL resistance and offers insights into the evolution of the mcr-1 gene in Salmonella. Frontiers Media S.A. 2020-12-22 /pmc/articles/PMC7782425/ /pubmed/33414775 http://dx.doi.org/10.3389/fmicb.2020.604710 Text en Copyright © 2020 Fan, Zhang, He, Zhao, Loh, Leptihn, Yu and Hua. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fan, Jianzhong
Zhang, Linghong
He, Jintao
Zhao, Maoying
Loh, Belinda
Leptihn, Sebastian
Yu, Yunsong
Hua, Xiaoting
Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title_full Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title_fullStr Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title_full_unstemmed Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title_short Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China
title_sort plasmid dynamics of mcr-1-positive salmonella spp. in a general hospital in china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782425/
https://www.ncbi.nlm.nih.gov/pubmed/33414775
http://dx.doi.org/10.3389/fmicb.2020.604710
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