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Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice

CONTEXT: The natural products derived from Capparis ecuadorica H.H. Iltis (Capparaceae) could have great potential for anti-inflammation since they inhibited the inflammatory response in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. OBJECT: This study investigated the anti-inflammatory effect...

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Autores principales: Song, Bo Ram, Lee, Su Jin, Kim, Ji Eun, Choi, Hyeon Jun, Bae, Su Ji, Choi, Yun Ju, Gong, Jeong Eun, Noh, Jin Kyung, Kim, Hye Sung, Kang, Hyun-Gu, Hong, Jin Tae, Hwang, Dae Youn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782699/
https://www.ncbi.nlm.nih.gov/pubmed/33355498
http://dx.doi.org/10.1080/13880209.2020.1856146
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author Song, Bo Ram
Lee, Su Jin
Kim, Ji Eun
Choi, Hyeon Jun
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Noh, Jin Kyung
Kim, Hye Sung
Kang, Hyun-Gu
Hong, Jin Tae
Hwang, Dae Youn
author_facet Song, Bo Ram
Lee, Su Jin
Kim, Ji Eun
Choi, Hyeon Jun
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Noh, Jin Kyung
Kim, Hye Sung
Kang, Hyun-Gu
Hong, Jin Tae
Hwang, Dae Youn
author_sort Song, Bo Ram
collection PubMed
description CONTEXT: The natural products derived from Capparis ecuadorica H.H. Iltis (Capparaceae) could have great potential for anti-inflammation since they inhibited the inflammatory response in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. OBJECT: This study investigated the anti-inflammatory effects and related mechanism of methanol extract of C. ecuadorica leaves (MCE) during atopic dermatitis (AD) responses. MATERIALS AND METHODS: Alterations in the phenotypical markers for AD, luciferase signal, iNOS‐mediated COX‐2 induction pathway, and inflammasome activation were analysed in non-Tg (n = 5) and 15% phthalic anhydride (PA) treated IL-4/Luc/CNS-1 transgenic (Tg) HR1 mice (n = 5 per group), subsequent to treatment with acetone-olive oil (AOO), vehicle (DMSO) and two dose MCE (20 and 40 mg/kg) three times a week for 4 weeks. RESULTS: MCE treatment reduced the intracellular ROS level (48.2%), NO concentration (7.1 mmol/L) and inflammatory cytokine expressions (39.1%) in the LPS-stimulated RAW264.7 cells. A significant decrease was detected for ear thickness (16.9%), weight of lymph node (0.7 mg), IgE concentration (1.9 µg/mL), and epidermal thickness (31.8%) of the PA + MCE treated Tg mice. MCE treatment induced the decrease of luciferase signal derived from the IL-4 promoter and the recovery of the IL-4 downstream regulator cytokines. PA + MCE treated Tg mice showed decreasing infiltration of mast cells (42.5%), iNOS-mediated COX‐2 induction pathway, MAPK signalling pathway and inflammasome activation in the ear tissue. CONCLUSIONS: These findings provide the first evidence that MCE may have great potential to suppress chemical-induced skin inflammation through the suppression of IL-4 cytokine and the iNOS-mediated COX‐2 induction pathway, and activation of inflammasome.
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spelling pubmed-77826992021-01-14 Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice Song, Bo Ram Lee, Su Jin Kim, Ji Eun Choi, Hyeon Jun Bae, Su Ji Choi, Yun Ju Gong, Jeong Eun Noh, Jin Kyung Kim, Hye Sung Kang, Hyun-Gu Hong, Jin Tae Hwang, Dae Youn Pharm Biol Research Article CONTEXT: The natural products derived from Capparis ecuadorica H.H. Iltis (Capparaceae) could have great potential for anti-inflammation since they inhibited the inflammatory response in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. OBJECT: This study investigated the anti-inflammatory effects and related mechanism of methanol extract of C. ecuadorica leaves (MCE) during atopic dermatitis (AD) responses. MATERIALS AND METHODS: Alterations in the phenotypical markers for AD, luciferase signal, iNOS‐mediated COX‐2 induction pathway, and inflammasome activation were analysed in non-Tg (n = 5) and 15% phthalic anhydride (PA) treated IL-4/Luc/CNS-1 transgenic (Tg) HR1 mice (n = 5 per group), subsequent to treatment with acetone-olive oil (AOO), vehicle (DMSO) and two dose MCE (20 and 40 mg/kg) three times a week for 4 weeks. RESULTS: MCE treatment reduced the intracellular ROS level (48.2%), NO concentration (7.1 mmol/L) and inflammatory cytokine expressions (39.1%) in the LPS-stimulated RAW264.7 cells. A significant decrease was detected for ear thickness (16.9%), weight of lymph node (0.7 mg), IgE concentration (1.9 µg/mL), and epidermal thickness (31.8%) of the PA + MCE treated Tg mice. MCE treatment induced the decrease of luciferase signal derived from the IL-4 promoter and the recovery of the IL-4 downstream regulator cytokines. PA + MCE treated Tg mice showed decreasing infiltration of mast cells (42.5%), iNOS-mediated COX‐2 induction pathway, MAPK signalling pathway and inflammasome activation in the ear tissue. CONCLUSIONS: These findings provide the first evidence that MCE may have great potential to suppress chemical-induced skin inflammation through the suppression of IL-4 cytokine and the iNOS-mediated COX‐2 induction pathway, and activation of inflammasome. Taylor & Francis 2020-12-23 /pmc/articles/PMC7782699/ /pubmed/33355498 http://dx.doi.org/10.1080/13880209.2020.1856146 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Song, Bo Ram
Lee, Su Jin
Kim, Ji Eun
Choi, Hyeon Jun
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Noh, Jin Kyung
Kim, Hye Sung
Kang, Hyun-Gu
Hong, Jin Tae
Hwang, Dae Youn
Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title_full Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title_fullStr Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title_full_unstemmed Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title_short Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice
title_sort anti-inflammatory effects of capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of il-4/luc/cns-1 transgenic mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782699/
https://www.ncbi.nlm.nih.gov/pubmed/33355498
http://dx.doi.org/10.1080/13880209.2020.1856146
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