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Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus
The diversity reflected by >100 different neural cell types fundamentally contributes to brain function and a central idea is that neuronal identity can be inferred from genetic information. Recent large-scale transcriptomic assays seem to confirm this hypothesis, but a lack of morphological info...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782706/ https://www.ncbi.nlm.nih.gov/pubmed/33398060 http://dx.doi.org/10.1038/s41467-020-20328-4 |
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author | Que, Lin Lukacsovich, David Luo, Wenshu Földy, Csaba |
author_facet | Que, Lin Lukacsovich, David Luo, Wenshu Földy, Csaba |
author_sort | Que, Lin |
collection | PubMed |
description | The diversity reflected by >100 different neural cell types fundamentally contributes to brain function and a central idea is that neuronal identity can be inferred from genetic information. Recent large-scale transcriptomic assays seem to confirm this hypothesis, but a lack of morphological information has limited the identification of several known cell types. In this study, we used single-cell RNA-seq in morphologically identified parvalbumin interneurons (PV-INs), and studied their transcriptomic states in the morphological, physiological, and developmental domains. Overall, we find high transcriptomic similarity among PV-INs, with few genes showing divergent expression between morphologically different types. Furthermore, PV-INs show a uniform synaptic cell adhesion molecule (CAM) profile, suggesting that CAM expression in mature PV cells does not reflect wiring specificity after development. Together, our results suggest that while PV-INs differ in anatomy and in vivo activity, their continuous transcriptomic and homogenous biophysical landscapes are not predictive of these distinct identities. |
format | Online Article Text |
id | pubmed-7782706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77827062021-01-11 Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus Que, Lin Lukacsovich, David Luo, Wenshu Földy, Csaba Nat Commun Article The diversity reflected by >100 different neural cell types fundamentally contributes to brain function and a central idea is that neuronal identity can be inferred from genetic information. Recent large-scale transcriptomic assays seem to confirm this hypothesis, but a lack of morphological information has limited the identification of several known cell types. In this study, we used single-cell RNA-seq in morphologically identified parvalbumin interneurons (PV-INs), and studied their transcriptomic states in the morphological, physiological, and developmental domains. Overall, we find high transcriptomic similarity among PV-INs, with few genes showing divergent expression between morphologically different types. Furthermore, PV-INs show a uniform synaptic cell adhesion molecule (CAM) profile, suggesting that CAM expression in mature PV cells does not reflect wiring specificity after development. Together, our results suggest that while PV-INs differ in anatomy and in vivo activity, their continuous transcriptomic and homogenous biophysical landscapes are not predictive of these distinct identities. Nature Publishing Group UK 2021-01-04 /pmc/articles/PMC7782706/ /pubmed/33398060 http://dx.doi.org/10.1038/s41467-020-20328-4 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Que, Lin Lukacsovich, David Luo, Wenshu Földy, Csaba Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title | Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title_full | Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title_fullStr | Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title_full_unstemmed | Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title_short | Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
title_sort | transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782706/ https://www.ncbi.nlm.nih.gov/pubmed/33398060 http://dx.doi.org/10.1038/s41467-020-20328-4 |
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