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Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N(2)-fixation in plants

Engineering nitrogen fixation in eukaryotes requires high expression of functional nitrogenase structural proteins, a goal that has not yet been achieved. Here we build a knowledge-based library containing 32 nitrogenase nifH sequences from prokaryotes of diverse ecological niches and metabolic feat...

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Detalles Bibliográficos
Autores principales: Jiang, Xi, Payá-Tormo, Lucía, Coroian, Diana, García-Rubio, Inés, Castellanos-Rueda, Rocío, Eseverri, Álvaro, López-Torrejón, Gema, Burén, Stefan, Rubio, Luis Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782807/
https://www.ncbi.nlm.nih.gov/pubmed/33398015
http://dx.doi.org/10.1038/s42003-020-01536-6