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Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782964/ https://www.ncbi.nlm.nih.gov/pubmed/33456722 http://dx.doi.org/10.1080/20002297.2020.1860398 |
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author | Chen, Juxiu Chen, Zhanyi Yuan, Keyong Huang, Zhengwei Mao, Mengying |
author_facet | Chen, Juxiu Chen, Zhanyi Yuan, Keyong Huang, Zhengwei Mao, Mengying |
author_sort | Chen, Juxiu |
collection | PubMed |
description | Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm formation of S. mutans. The T4 Rnl1 mutant exhibited similar growth phenotype but resulted in a significant reduction of biofilm biomass compared to wild type strain and empty plasmid carrying strain. The abnormal biofilm of the T4 Rnl1 mutant harbored loose bacterial clusters with defective production and distribution of exopolysaccharides. Moreover, the expression of several biofilm formation-associated genes was dysregulated at mRNA level in the T4 Rnl1 mutant. These results reveal that the bacteriophage T4 Rnl1 exert antibiofilm activities against the cariogenic bacterium S. mutans, which impacts the spatial organization of the exopolysaccharides and further impairs the three-dimensional biofilm architecture. These findings implicate that manipulation of bacteriophage T4 Rnl1, a biological tool used for RNA ligation, will provide a promising approach to cariogenic biofilm control. |
format | Online Article Text |
id | pubmed-7782964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-77829642021-01-14 Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation Chen, Juxiu Chen, Zhanyi Yuan, Keyong Huang, Zhengwei Mao, Mengying J Oral Microbiol Original Article Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm formation of S. mutans. The T4 Rnl1 mutant exhibited similar growth phenotype but resulted in a significant reduction of biofilm biomass compared to wild type strain and empty plasmid carrying strain. The abnormal biofilm of the T4 Rnl1 mutant harbored loose bacterial clusters with defective production and distribution of exopolysaccharides. Moreover, the expression of several biofilm formation-associated genes was dysregulated at mRNA level in the T4 Rnl1 mutant. These results reveal that the bacteriophage T4 Rnl1 exert antibiofilm activities against the cariogenic bacterium S. mutans, which impacts the spatial organization of the exopolysaccharides and further impairs the three-dimensional biofilm architecture. These findings implicate that manipulation of bacteriophage T4 Rnl1, a biological tool used for RNA ligation, will provide a promising approach to cariogenic biofilm control. Taylor & Francis 2020-12-24 /pmc/articles/PMC7782964/ /pubmed/33456722 http://dx.doi.org/10.1080/20002297.2020.1860398 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Chen, Juxiu Chen, Zhanyi Yuan, Keyong Huang, Zhengwei Mao, Mengying Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title | Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title_full | Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title_fullStr | Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title_full_unstemmed | Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title_short | Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation |
title_sort | recombinant bacteriophage t4 rnl1 impacts streptococcus mutans biofilm formation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782964/ https://www.ncbi.nlm.nih.gov/pubmed/33456722 http://dx.doi.org/10.1080/20002297.2020.1860398 |
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