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Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation

Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pa...

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Autores principales: Chen, Juxiu, Chen, Zhanyi, Yuan, Keyong, Huang, Zhengwei, Mao, Mengying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782964/
https://www.ncbi.nlm.nih.gov/pubmed/33456722
http://dx.doi.org/10.1080/20002297.2020.1860398
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author Chen, Juxiu
Chen, Zhanyi
Yuan, Keyong
Huang, Zhengwei
Mao, Mengying
author_facet Chen, Juxiu
Chen, Zhanyi
Yuan, Keyong
Huang, Zhengwei
Mao, Mengying
author_sort Chen, Juxiu
collection PubMed
description Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm formation of S. mutans. The T4 Rnl1 mutant exhibited similar growth phenotype but resulted in a significant reduction of biofilm biomass compared to wild type strain and empty plasmid carrying strain. The abnormal biofilm of the T4 Rnl1 mutant harbored loose bacterial clusters with defective production and distribution of exopolysaccharides. Moreover, the expression of several biofilm formation-associated genes was dysregulated at mRNA level in the T4 Rnl1 mutant. These results reveal that the bacteriophage T4 Rnl1 exert antibiofilm activities against the cariogenic bacterium S. mutans, which impacts the spatial organization of the exopolysaccharides and further impairs the three-dimensional biofilm architecture. These findings implicate that manipulation of bacteriophage T4 Rnl1, a biological tool used for RNA ligation, will provide a promising approach to cariogenic biofilm control.
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spelling pubmed-77829642021-01-14 Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation Chen, Juxiu Chen, Zhanyi Yuan, Keyong Huang, Zhengwei Mao, Mengying J Oral Microbiol Original Article Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm formation of S. mutans. The T4 Rnl1 mutant exhibited similar growth phenotype but resulted in a significant reduction of biofilm biomass compared to wild type strain and empty plasmid carrying strain. The abnormal biofilm of the T4 Rnl1 mutant harbored loose bacterial clusters with defective production and distribution of exopolysaccharides. Moreover, the expression of several biofilm formation-associated genes was dysregulated at mRNA level in the T4 Rnl1 mutant. These results reveal that the bacteriophage T4 Rnl1 exert antibiofilm activities against the cariogenic bacterium S. mutans, which impacts the spatial organization of the exopolysaccharides and further impairs the three-dimensional biofilm architecture. These findings implicate that manipulation of bacteriophage T4 Rnl1, a biological tool used for RNA ligation, will provide a promising approach to cariogenic biofilm control. Taylor & Francis 2020-12-24 /pmc/articles/PMC7782964/ /pubmed/33456722 http://dx.doi.org/10.1080/20002297.2020.1860398 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chen, Juxiu
Chen, Zhanyi
Yuan, Keyong
Huang, Zhengwei
Mao, Mengying
Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title_full Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title_fullStr Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title_full_unstemmed Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title_short Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation
title_sort recombinant bacteriophage t4 rnl1 impacts streptococcus mutans biofilm formation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782964/
https://www.ncbi.nlm.nih.gov/pubmed/33456722
http://dx.doi.org/10.1080/20002297.2020.1860398
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