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An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line
Oral squamous cell carcinoma (OSCC) is the most commom cancer in the world. If remain untreated for several years, it may be fatal. Hence, it is important to prevent and treat OSCC at an early stage. In this study the effect of aqueous and dry leaves extract of Ocimum sanctum was observed on Ca9-22...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783796/ https://www.ncbi.nlm.nih.gov/pubmed/33424380 http://dx.doi.org/10.1016/j.sjbs.2020.11.030 |
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author | Luke, A.M. Patnaik, R. Kuriadom, S.T. Jaber, M. Mathew, S. |
author_facet | Luke, A.M. Patnaik, R. Kuriadom, S.T. Jaber, M. Mathew, S. |
author_sort | Luke, A.M. |
collection | PubMed |
description | Oral squamous cell carcinoma (OSCC) is the most commom cancer in the world. If remain untreated for several years, it may be fatal. Hence, it is important to prevent and treat OSCC at an early stage. In this study the effect of aqueous and dry leaves extract of Ocimum sanctum was observed on Ca9-22 cell line, which is an OSCC cell line. For this, Ca9-22 cell line was cultured and maintained. After 24 h, the cells were treated with aqueous and dry leaves extract of Ocimum sanctum plant. Viability of the cancerous cells were studied by 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and neutral red uptake (NRU) assay. Minimum inhibitory concentrations (MIC), lethal concentration(25) (LC(25)), lethal concentration(50) (LC(50)) and highest permissive concentration (HPC) was calculated by probit computational method. Experimentally, the MIC value was 5 mg/L, whereas the HPC was 30 mg/L of the plant extract in aqueous state. For the dry extract the MIC was 5 mg/L whereas the HPC was 35 mg/L for both MTT and NRU assays. For MTT assay LC values: 7.41 (LC(25)), 14.79 (LC(50)) and 26.91 mg/L (LC(75)) for aqueous extract and 12.58 (LC(25)), 20.89 (LC(50)), 29.51 mg/L (LC(75)) for dry extract. For NRU assay LC values were 10.23 (LC(25)), 14.79 (LC(50)) and 20.89 mg/L (LC(75)) aqueous extract, and 16.59 (LC(25)), 23.44 (LC(50)), 30.19 mg/L (LC(75)) dry extract of the plant. From the above study it was concluded that, Ocimum sanctum have anti-cancerous activity. It can further be used for therapeutic purposes. |
format | Online Article Text |
id | pubmed-7783796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77837962021-01-08 An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line Luke, A.M. Patnaik, R. Kuriadom, S.T. Jaber, M. Mathew, S. Saudi J Biol Sci Original Article Oral squamous cell carcinoma (OSCC) is the most commom cancer in the world. If remain untreated for several years, it may be fatal. Hence, it is important to prevent and treat OSCC at an early stage. In this study the effect of aqueous and dry leaves extract of Ocimum sanctum was observed on Ca9-22 cell line, which is an OSCC cell line. For this, Ca9-22 cell line was cultured and maintained. After 24 h, the cells were treated with aqueous and dry leaves extract of Ocimum sanctum plant. Viability of the cancerous cells were studied by 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and neutral red uptake (NRU) assay. Minimum inhibitory concentrations (MIC), lethal concentration(25) (LC(25)), lethal concentration(50) (LC(50)) and highest permissive concentration (HPC) was calculated by probit computational method. Experimentally, the MIC value was 5 mg/L, whereas the HPC was 30 mg/L of the plant extract in aqueous state. For the dry extract the MIC was 5 mg/L whereas the HPC was 35 mg/L for both MTT and NRU assays. For MTT assay LC values: 7.41 (LC(25)), 14.79 (LC(50)) and 26.91 mg/L (LC(75)) for aqueous extract and 12.58 (LC(25)), 20.89 (LC(50)), 29.51 mg/L (LC(75)) for dry extract. For NRU assay LC values were 10.23 (LC(25)), 14.79 (LC(50)) and 20.89 mg/L (LC(75)) aqueous extract, and 16.59 (LC(25)), 23.44 (LC(50)), 30.19 mg/L (LC(75)) dry extract of the plant. From the above study it was concluded that, Ocimum sanctum have anti-cancerous activity. It can further be used for therapeutic purposes. Elsevier 2021-01 2020-11-11 /pmc/articles/PMC7783796/ /pubmed/33424380 http://dx.doi.org/10.1016/j.sjbs.2020.11.030 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Luke, A.M. Patnaik, R. Kuriadom, S.T. Jaber, M. Mathew, S. An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title | An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title_full | An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title_fullStr | An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title_full_unstemmed | An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title_short | An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
title_sort | in vitro study of ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783796/ https://www.ncbi.nlm.nih.gov/pubmed/33424380 http://dx.doi.org/10.1016/j.sjbs.2020.11.030 |
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