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Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti
BACKGROUND: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants, Pithecellobium dulce (Fabaceae), Le...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783993/ https://www.ncbi.nlm.nih.gov/pubmed/33397448 http://dx.doi.org/10.1186/s13071-020-04519-y |
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author | Nyasembe, Vincent Odhiambo Tchouassi, David Poumo Muturi, Martha Njeri Pirk, Christian W. W. Sole, Catherine L. Torto, Baldwyn |
author_facet | Nyasembe, Vincent Odhiambo Tchouassi, David Poumo Muturi, Martha Njeri Pirk, Christian W. W. Sole, Catherine L. Torto, Baldwyn |
author_sort | Nyasembe, Vincent Odhiambo |
collection | PubMed |
description | BACKGROUND: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants, Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae), on the survival, fecundity, and egg viability of the dengue vector Aedes aegypti. METHODS: We tested these effects using females that received (i) an initial three rations of blood meals and (ii) no blood meal at all. Two controls were included: age-matched females fed on glucose solution with or without an initial blood meal and those fed exclusively on blood meals. Data were collected daily over a 30-day period. The amino acid contents of Ae. aegypti guts and their respective diets were detected by coupled liquid chromatography-mass spectrometry. RESULTS: Females fed on P. dulce and an exclusively blood meal diet had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on blood meals, whereas those fed on O. ficus-indica had the shortest survival time. With an initial blood meal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs compared to those fed exclusively on blood meals. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. CONCLUSION: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors, which may impact their disease transmission potential. [Image: see text] |
format | Online Article Text |
id | pubmed-7783993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77839932021-01-14 Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti Nyasembe, Vincent Odhiambo Tchouassi, David Poumo Muturi, Martha Njeri Pirk, Christian W. W. Sole, Catherine L. Torto, Baldwyn Parasit Vectors Research BACKGROUND: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants, Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae), on the survival, fecundity, and egg viability of the dengue vector Aedes aegypti. METHODS: We tested these effects using females that received (i) an initial three rations of blood meals and (ii) no blood meal at all. Two controls were included: age-matched females fed on glucose solution with or without an initial blood meal and those fed exclusively on blood meals. Data were collected daily over a 30-day period. The amino acid contents of Ae. aegypti guts and their respective diets were detected by coupled liquid chromatography-mass spectrometry. RESULTS: Females fed on P. dulce and an exclusively blood meal diet had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on blood meals, whereas those fed on O. ficus-indica had the shortest survival time. With an initial blood meal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs compared to those fed exclusively on blood meals. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. CONCLUSION: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors, which may impact their disease transmission potential. [Image: see text] BioMed Central 2021-01-04 /pmc/articles/PMC7783993/ /pubmed/33397448 http://dx.doi.org/10.1186/s13071-020-04519-y Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Nyasembe, Vincent Odhiambo Tchouassi, David Poumo Muturi, Martha Njeri Pirk, Christian W. W. Sole, Catherine L. Torto, Baldwyn Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title | Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title_full | Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title_fullStr | Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title_full_unstemmed | Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title_short | Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti |
title_sort | plant nutrient quality impacts survival and reproductive fitness of the dengue vector aedes aegypti |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783993/ https://www.ncbi.nlm.nih.gov/pubmed/33397448 http://dx.doi.org/10.1186/s13071-020-04519-y |
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