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Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7
BACKGROUND: Leptin Receptor (LEPR) has been suggested to have several roles in cancer metastasis. However, the role of LEPR and its underlying mechanisms in lymphatic metastasis of hepatocarcinoma have not yet been studied. METHODS: We performed bioinformatics analysis, qRT-PCR, western blotting, im...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784271/ https://www.ncbi.nlm.nih.gov/pubmed/33397392 http://dx.doi.org/10.1186/s12935-020-01641-w |
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author | Huang, He Zhang, Jun Ling, Fei Huang, Yuhong Yang, Min Zhang, Yao Wei, Yuanyi Zhang, Qingqing Wang, Honghai Song, Lin Wu, Ying Yang, Jiayu Tang, Jianwu |
author_facet | Huang, He Zhang, Jun Ling, Fei Huang, Yuhong Yang, Min Zhang, Yao Wei, Yuanyi Zhang, Qingqing Wang, Honghai Song, Lin Wu, Ying Yang, Jiayu Tang, Jianwu |
author_sort | Huang, He |
collection | PubMed |
description | BACKGROUND: Leptin Receptor (LEPR) has been suggested to have several roles in cancer metastasis. However, the role of LEPR and its underlying mechanisms in lymphatic metastasis of hepatocarcinoma have not yet been studied. METHODS: We performed bioinformatics analysis, qRT-PCR, western blotting, immunohistochemistry, immunofluorescence, enzyme-linked immunosorbent, coimmunoprecipitation assays and a series of functional assays to investigate the roles of LEPR in hepatocellular carcinoma. RESULTS: We discovered that LEPR was highly expressed in liver cancer tissues, and the expression of LEPR in Hca-F cells was higher than that in Hca-P cells. Furthermore, LEPR promotes the proliferation, migration and invasion and inhibits the apoptosis of hepatocarcinoma lymphatic metastatic cells. Further studies indicated that LEPR interacts with ANXA7. Mechanistically, LEPR regulated ERK1/2 and JAK2/STAT3 expression via ANXA7 regulation. CONCLUSIONS: These findings unveiled a previously unappreciated role of LEPR in the regulation of lymphatic metastatic hepatocellular carcinoma, assigning ANXA7-LEPR as a promising therapeutic target for liver cancer treatments. |
format | Online Article Text |
id | pubmed-7784271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77842712021-01-14 Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 Huang, He Zhang, Jun Ling, Fei Huang, Yuhong Yang, Min Zhang, Yao Wei, Yuanyi Zhang, Qingqing Wang, Honghai Song, Lin Wu, Ying Yang, Jiayu Tang, Jianwu Cancer Cell Int Primary Research BACKGROUND: Leptin Receptor (LEPR) has been suggested to have several roles in cancer metastasis. However, the role of LEPR and its underlying mechanisms in lymphatic metastasis of hepatocarcinoma have not yet been studied. METHODS: We performed bioinformatics analysis, qRT-PCR, western blotting, immunohistochemistry, immunofluorescence, enzyme-linked immunosorbent, coimmunoprecipitation assays and a series of functional assays to investigate the roles of LEPR in hepatocellular carcinoma. RESULTS: We discovered that LEPR was highly expressed in liver cancer tissues, and the expression of LEPR in Hca-F cells was higher than that in Hca-P cells. Furthermore, LEPR promotes the proliferation, migration and invasion and inhibits the apoptosis of hepatocarcinoma lymphatic metastatic cells. Further studies indicated that LEPR interacts with ANXA7. Mechanistically, LEPR regulated ERK1/2 and JAK2/STAT3 expression via ANXA7 regulation. CONCLUSIONS: These findings unveiled a previously unappreciated role of LEPR in the regulation of lymphatic metastatic hepatocellular carcinoma, assigning ANXA7-LEPR as a promising therapeutic target for liver cancer treatments. BioMed Central 2021-01-04 /pmc/articles/PMC7784271/ /pubmed/33397392 http://dx.doi.org/10.1186/s12935-020-01641-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Huang, He Zhang, Jun Ling, Fei Huang, Yuhong Yang, Min Zhang, Yao Wei, Yuanyi Zhang, Qingqing Wang, Honghai Song, Lin Wu, Ying Yang, Jiayu Tang, Jianwu Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title | Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title_full | Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title_fullStr | Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title_full_unstemmed | Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title_short | Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7 |
title_sort | leptin receptor (lepr) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating anxa7 |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784271/ https://www.ncbi.nlm.nih.gov/pubmed/33397392 http://dx.doi.org/10.1186/s12935-020-01641-w |
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