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Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4

BACKGROUND: Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing of the bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine the impact of number of polymerase chain reaction (PCR) steps (1- or 2- steps) and cho...

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Detalles Bibliográficos
Autores principales:	Drengenes, Christine, Eagan, Tomas M. L., Haaland, Ingvild, Wiker, Harald G., Nielsen, Rune
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784388/ https://www.ncbi.nlm.nih.gov/pubmed/33397283 http://dx.doi.org/10.1186/s12864-020-07252-z

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