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A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas

Gliomas are one of the most aggressive primary brain tumors arising from neural progenitor cells. Delayed diagnosis, invasive biopsy, and diagnostic challenges stems the need for specific, minimally-invasive, and early diagnostic biomarkers. Tumor-associated (TA) autoantibodies are measurable in the...

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Autores principales: Gahoi, Nikita, Syed, Parvez, Choudhary, Saket, Epari, Sridhar, Moiyadi, Aliasgar, Varma, Santosh G., Gandhi, Mayuri N., Srivastava, Sanjeeva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784397/
https://www.ncbi.nlm.nih.gov/pubmed/33415070
http://dx.doi.org/10.3389/fonc.2020.543947
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author Gahoi, Nikita
Syed, Parvez
Choudhary, Saket
Epari, Sridhar
Moiyadi, Aliasgar
Varma, Santosh G.
Gandhi, Mayuri N.
Srivastava, Sanjeeva
author_facet Gahoi, Nikita
Syed, Parvez
Choudhary, Saket
Epari, Sridhar
Moiyadi, Aliasgar
Varma, Santosh G.
Gandhi, Mayuri N.
Srivastava, Sanjeeva
author_sort Gahoi, Nikita
collection PubMed
description Gliomas are one of the most aggressive primary brain tumors arising from neural progenitor cells. Delayed diagnosis, invasive biopsy, and diagnostic challenges stems the need for specific, minimally-invasive, and early diagnostic biomarkers. Tumor-associated (TA) autoantibodies are measurable in the biofluids long before the onset of the symptoms, suggesting their role in early diagnosis and clinical management of the patients. In the current study, cerebrospinal fluid (CSF) samples from patients with low-grade glioma (LGG) and the Glioblastoma multiforme (GBM) that characterizes advanced disease were compared with healthy control samples to identify putative TA autoantibodies, using protein microarrays. The CSF samples from LGGs (n = 10), GBM (n = 7) were compared with the control CSF samples (n = 6). Proteins showing significant antigenic response were cross-verified. Proteins NOL4 (a cancer-testis antigen) and KALRN showed an antigenic response in the CSF of GBM patients, whereas, UTP4 and CCDC28A showed an antigenic response in low grade gliomas when compared with the control samples. TA autoantibodies identified in this study from the CSF of the patients could supplement current screening modalities. Further validation of these TA autoantibodies on a larger clinical cohort could provide cues towards relevance of these proteins in early diagnosis of the disease.
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spelling pubmed-77843972021-01-06 A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas Gahoi, Nikita Syed, Parvez Choudhary, Saket Epari, Sridhar Moiyadi, Aliasgar Varma, Santosh G. Gandhi, Mayuri N. Srivastava, Sanjeeva Front Oncol Oncology Gliomas are one of the most aggressive primary brain tumors arising from neural progenitor cells. Delayed diagnosis, invasive biopsy, and diagnostic challenges stems the need for specific, minimally-invasive, and early diagnostic biomarkers. Tumor-associated (TA) autoantibodies are measurable in the biofluids long before the onset of the symptoms, suggesting their role in early diagnosis and clinical management of the patients. In the current study, cerebrospinal fluid (CSF) samples from patients with low-grade glioma (LGG) and the Glioblastoma multiforme (GBM) that characterizes advanced disease were compared with healthy control samples to identify putative TA autoantibodies, using protein microarrays. The CSF samples from LGGs (n = 10), GBM (n = 7) were compared with the control CSF samples (n = 6). Proteins showing significant antigenic response were cross-verified. Proteins NOL4 (a cancer-testis antigen) and KALRN showed an antigenic response in the CSF of GBM patients, whereas, UTP4 and CCDC28A showed an antigenic response in low grade gliomas when compared with the control samples. TA autoantibodies identified in this study from the CSF of the patients could supplement current screening modalities. Further validation of these TA autoantibodies on a larger clinical cohort could provide cues towards relevance of these proteins in early diagnosis of the disease. Frontiers Media S.A. 2020-12-22 /pmc/articles/PMC7784397/ /pubmed/33415070 http://dx.doi.org/10.3389/fonc.2020.543947 Text en Copyright © 2020 Gahoi, Syed, Choudhary, Epari, Moiyadi, Varma, Gandhi and Srivastava http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Gahoi, Nikita
Syed, Parvez
Choudhary, Saket
Epari, Sridhar
Moiyadi, Aliasgar
Varma, Santosh G.
Gandhi, Mayuri N.
Srivastava, Sanjeeva
A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title_full A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title_fullStr A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title_full_unstemmed A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title_short A Protein Microarray-Based Investigation of Cerebrospinal Fluid Reveals Distinct Autoantibody Signature in Low and High-Grade Gliomas
title_sort protein microarray-based investigation of cerebrospinal fluid reveals distinct autoantibody signature in low and high-grade gliomas
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784397/
https://www.ncbi.nlm.nih.gov/pubmed/33415070
http://dx.doi.org/10.3389/fonc.2020.543947
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