The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound

We studied the paracrine function of mesenchymal stem cells (MSCs) derived from various sources in response to pulsed focused ultrasound (pFUS). Human adipose tissue (AD), bone marrow (BM), and umbilical cord (UC) derived MSCs were exposed to pFUS at two intensities: 0.45 W/cm(2) I(SATA) (310 kPa PN...

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Detalles Bibliográficos
Autores principales: Razavi, Mehdi, Rezaee, Melika, Telichko, Arsenii, Inan, Hakan, Dahl, Jeremy, Demirci, Utkan, Thakor, Avnesh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784560/
https://www.ncbi.nlm.nih.gov/pubmed/33028105
http://dx.doi.org/10.1177/0963689720965478
Descripción
Sumario:We studied the paracrine function of mesenchymal stem cells (MSCs) derived from various sources in response to pulsed focused ultrasound (pFUS). Human adipose tissue (AD), bone marrow (BM), and umbilical cord (UC) derived MSCs were exposed to pFUS at two intensities: 0.45 W/cm(2) I(SATA) (310 kPa PNP) and 1.3 W/cm(2) I(SATA) (540 kPa PNP). Following pFUS, the viability and proliferation of MSCs were assessed using a hemocytometer and confocal microscopy, and their secreted cytokine profile determined using a multiplex ELISA. Our findings showed that pFUS can stimulate the production of immunomodulatory, anti-inflammatory, and angiogenic cytokines from MSCs which was dependent on both the source of MSC being studied and the acoustic intensity employed. These important findings set the foundation for additional mechanistic and validation studies using this novel noninvasive and clinically translatable technology for modulating MSC biology.

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