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Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes

Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovas...

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Autores principales: Ogay, Vyacheslav, Kumasheva, Venera, Li, Yelena, Mukhlis, Sholpan, Sekenova, Aliya, Olzhayev, Farkhad, Tsoy, Andrey, Umbayev, Baurzhan, Askarova, Sholpan, Shpekov, Azat, Kaliyev, Assylbek, Zhetpisbayev, Berik, Makhambetov, Yerbol, Akshulakov, Serik, Saparov, Arman, Ramankulov, Yerlan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784564/
https://www.ncbi.nlm.nih.gov/pubmed/32885682
http://dx.doi.org/10.1177/0963689720956956
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author Ogay, Vyacheslav
Kumasheva, Venera
Li, Yelena
Mukhlis, Sholpan
Sekenova, Aliya
Olzhayev, Farkhad
Tsoy, Andrey
Umbayev, Baurzhan
Askarova, Sholpan
Shpekov, Azat
Kaliyev, Assylbek
Zhetpisbayev, Berik
Makhambetov, Yerbol
Akshulakov, Serik
Saparov, Arman
Ramankulov, Yerlan
author_facet Ogay, Vyacheslav
Kumasheva, Venera
Li, Yelena
Mukhlis, Sholpan
Sekenova, Aliya
Olzhayev, Farkhad
Tsoy, Andrey
Umbayev, Baurzhan
Askarova, Sholpan
Shpekov, Azat
Kaliyev, Assylbek
Zhetpisbayev, Berik
Makhambetov, Yerbol
Akshulakov, Serik
Saparov, Arman
Ramankulov, Yerlan
author_sort Ogay, Vyacheslav
collection PubMed
description Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were isolated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neurogenesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline–treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.
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spelling pubmed-77845642021-01-14 Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes Ogay, Vyacheslav Kumasheva, Venera Li, Yelena Mukhlis, Sholpan Sekenova, Aliya Olzhayev, Farkhad Tsoy, Andrey Umbayev, Baurzhan Askarova, Sholpan Shpekov, Azat Kaliyev, Assylbek Zhetpisbayev, Berik Makhambetov, Yerbol Akshulakov, Serik Saparov, Arman Ramankulov, Yerlan Cell Transplant Original Article Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were isolated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neurogenesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline–treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients. SAGE Publications 2020-09-04 /pmc/articles/PMC7784564/ /pubmed/32885682 http://dx.doi.org/10.1177/0963689720956956 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Ogay, Vyacheslav
Kumasheva, Venera
Li, Yelena
Mukhlis, Sholpan
Sekenova, Aliya
Olzhayev, Farkhad
Tsoy, Andrey
Umbayev, Baurzhan
Askarova, Sholpan
Shpekov, Azat
Kaliyev, Assylbek
Zhetpisbayev, Berik
Makhambetov, Yerbol
Akshulakov, Serik
Saparov, Arman
Ramankulov, Yerlan
Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title_full Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title_fullStr Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title_full_unstemmed Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title_short Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes
title_sort improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784564/
https://www.ncbi.nlm.nih.gov/pubmed/32885682
http://dx.doi.org/10.1177/0963689720956956
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