Protocol to select conformation-specific intracellular antibodies for targeted protein degradation in an engineered cell line

Here, we provide a protocol for the selection of conformation-specific intracellular antibody degraders using a cell-based screening method. We applied this protocol to select antibody-based degraders targeting the active form of the small GTPase RHOB (i.e., RHOB-GTP) using an engineered H2882 cell...

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Detalles Bibliográficos
Autores principales: Bery, Nicolas, Favre, Gilles, Olichon, Aurélien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786126/
https://www.ncbi.nlm.nih.gov/pubmed/33437969
http://dx.doi.org/10.1016/j.xpro.2020.100249

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786126/
https://www.ncbi.nlm.nih.gov/pubmed/33437969
http://dx.doi.org/10.1016/j.xpro.2020.100249

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