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Protocol to select conformation-specific intracellular antibodies for targeted protein degradation in an engineered cell line
Here, we provide a protocol for the selection of conformation-specific intracellular antibody degraders using a cell-based screening method. We applied this protocol to select antibody-based degraders targeting the active form of the small GTPase RHOB (i.e., RHOB-GTP) using an engineered H2882 cell...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786126/ https://www.ncbi.nlm.nih.gov/pubmed/33437969 http://dx.doi.org/10.1016/j.xpro.2020.100249 |