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Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis
Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. Xenopus laevis embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Caltech Library
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786221/ https://www.ncbi.nlm.nih.gov/pubmed/33426508 http://dx.doi.org/10.17912/micropub.biology.000347 |
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author | Colozza, Gabriele |
author_facet | Colozza, Gabriele |
author_sort | Colozza, Gabriele |
collection | PubMed |
description | Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. Xenopus laevis embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating embryonic axis development, and modulation of the Wnt pathway is required for proper antero-posterior patterning. Recently, a novel secreted, organizer-specific Wnt inhibitor, Bighead, was identified that acts by downregulating Lrp6 plasma membrane levels. Here, I describe a method to purify biologically active Bighead protein and confirm that Bighead promotes Xenopus head development. |
format | Online Article Text |
id | pubmed-7786221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-77862212021-01-07 Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis Colozza, Gabriele MicroPubl Biol New Finding Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. Xenopus laevis embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating embryonic axis development, and modulation of the Wnt pathway is required for proper antero-posterior patterning. Recently, a novel secreted, organizer-specific Wnt inhibitor, Bighead, was identified that acts by downregulating Lrp6 plasma membrane levels. Here, I describe a method to purify biologically active Bighead protein and confirm that Bighead promotes Xenopus head development. Caltech Library 2021-01-05 /pmc/articles/PMC7786221/ /pubmed/33426508 http://dx.doi.org/10.17912/micropub.biology.000347 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Colozza, Gabriele Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title | Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title_full | Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title_fullStr | Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title_full_unstemmed | Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title_short | Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis |
title_sort | purified bighead protein efficiently promotes head development in the south african clawed frog, xenopus laevis |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786221/ https://www.ncbi.nlm.nih.gov/pubmed/33426508 http://dx.doi.org/10.17912/micropub.biology.000347 |
work_keys_str_mv | AT colozzagabriele purifiedbigheadproteinefficientlypromotesheaddevelopmentinthesouthafricanclawedfrogxenopuslaevis |