Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3

Conditional knockout (cKO) mice have contributed greatly to understanding the tissue- or stage-specific functions of genes in vivo. However, the current cKO method requires considerable time and effort because of the need to generate two gene-modified mouse strains (Cre transgenic and loxP knockin)...

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Detalles Bibliográficos
Autores principales: Miura, Kento, Matoba, Shogo, Hirose, Michiko, Ogura, Atsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786261/
https://www.ncbi.nlm.nih.gov/pubmed/32965494
http://dx.doi.org/10.1093/biolre/ioaa176

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786261/
https://www.ncbi.nlm.nih.gov/pubmed/32965494
http://dx.doi.org/10.1093/biolre/ioaa176

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