RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts

The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA‐Seq gene expression, quantitative protein expression, and siRNA knockdown we identified genes and their cognate proteins which are similarly...

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Detalles Bibliográficos
Autores principales: Azar, Christopher, Valentine, Mark C., Trausch‐Azar, Julie, Rois, Lisa, Mahjoub, Moe, Nelson, D. Michael, Schwartz, Alan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786548/
https://www.ncbi.nlm.nih.gov/pubmed/33403800
http://dx.doi.org/10.14814/phy2.14671
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author Azar, Christopher
Valentine, Mark C.
Trausch‐Azar, Julie
Rois, Lisa
Mahjoub, Moe
Nelson, D. Michael
Schwartz, Alan L.
author_facet Azar, Christopher
Valentine, Mark C.
Trausch‐Azar, Julie
Rois, Lisa
Mahjoub, Moe
Nelson, D. Michael
Schwartz, Alan L.
author_sort Azar, Christopher
collection PubMed
description The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA‐Seq gene expression, quantitative protein expression, and siRNA knockdown we identified genes and their cognate proteins which are similarly upregulated in two cellular models of mammalian syncytia development (human BeWo cytotrophoblast to syncytiotrophoblast and murine C2C12 myoblast to myotube). These include DYSF, PDE4DIP, SPIRE2, NDRG1, PLEC, GPR146, HSPB8, DHCR7, and HDAC5. These findings provide avenues for further understanding of the mechanisms underlying mammalian placental syncytiotrophoblast development.
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spelling pubmed-77865482021-01-11 RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts Azar, Christopher Valentine, Mark C. Trausch‐Azar, Julie Rois, Lisa Mahjoub, Moe Nelson, D. Michael Schwartz, Alan L. Physiol Rep Original Research The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA‐Seq gene expression, quantitative protein expression, and siRNA knockdown we identified genes and their cognate proteins which are similarly upregulated in two cellular models of mammalian syncytia development (human BeWo cytotrophoblast to syncytiotrophoblast and murine C2C12 myoblast to myotube). These include DYSF, PDE4DIP, SPIRE2, NDRG1, PLEC, GPR146, HSPB8, DHCR7, and HDAC5. These findings provide avenues for further understanding of the mechanisms underlying mammalian placental syncytiotrophoblast development. John Wiley and Sons Inc. 2021-01-06 /pmc/articles/PMC7786548/ /pubmed/33403800 http://dx.doi.org/10.14814/phy2.14671 Text en © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Azar, Christopher
Valentine, Mark C.
Trausch‐Azar, Julie
Rois, Lisa
Mahjoub, Moe
Nelson, D. Michael
Schwartz, Alan L.
RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title_full RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title_fullStr RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title_full_unstemmed RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title_short RNA‐Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
title_sort rna‐seq identifies genes whose proteins are upregulated during syncytia development in murine c2c12 myoblasts and human bewo trophoblasts
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786548/
https://www.ncbi.nlm.nih.gov/pubmed/33403800
http://dx.doi.org/10.14814/phy2.14671
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