Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies

Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes...

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Autores principales: Bai, Manyuan, Wang, Rui, Sun, Shiqi, Zhang, Yun, Dong, Hu, Guo, Huichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787412/
https://www.ncbi.nlm.nih.gov/pubmed/33409664
http://dx.doi.org/10.1186/s13568-020-01167-4
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author Bai, Manyuan
Wang, Rui
Sun, Shiqi
Zhang, Yun
Dong, Hu
Guo, Huichen
author_facet Bai, Manyuan
Wang, Rui
Sun, Shiqi
Zhang, Yun
Dong, Hu
Guo, Huichen
author_sort Bai, Manyuan
collection PubMed
description Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLPs-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLPs-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples.
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spelling pubmed-77874122021-01-07 Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies Bai, Manyuan Wang, Rui Sun, Shiqi Zhang, Yun Dong, Hu Guo, Huichen AMB Express Original Article Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLPs-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLPs-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples. Springer Berlin Heidelberg 2021-01-06 /pmc/articles/PMC7787412/ /pubmed/33409664 http://dx.doi.org/10.1186/s13568-020-01167-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Bai, Manyuan
Wang, Rui
Sun, Shiqi
Zhang, Yun
Dong, Hu
Guo, Huichen
Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title_full Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title_fullStr Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title_full_unstemmed Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title_short Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies
title_sort development and validation of a competitive elisa based on virus-like particles of serotype senecavirus a to detect serum antibodies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787412/
https://www.ncbi.nlm.nih.gov/pubmed/33409664
http://dx.doi.org/10.1186/s13568-020-01167-4
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