Cargando…
Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis
PURPOSE: Jie-Du-Hua-Yu (JDHY) granules are a traditional Chinese medicine with known therapeutic effects for the treatment of acute liver failure (ALF). This study explored the potential molecular mechanism(s) of JDHY granules in promoting liver regeneration and preventing ALF. METHODS: Rat models o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787748/ https://www.ncbi.nlm.nih.gov/pubmed/33456491 http://dx.doi.org/10.1155/2020/8180959 |
_version_ | 1783632887564730368 |
---|---|
author | Wang, Tingshuai Wang, Na Zhang, Rongzhen Huang, Shaodong Qiu, Hua Long, Fuli Wang, Minggang Mao, Dewen |
author_facet | Wang, Tingshuai Wang, Na Zhang, Rongzhen Huang, Shaodong Qiu, Hua Long, Fuli Wang, Minggang Mao, Dewen |
author_sort | Wang, Tingshuai |
collection | PubMed |
description | PURPOSE: Jie-Du-Hua-Yu (JDHY) granules are a traditional Chinese medicine with known therapeutic effects for the treatment of acute liver failure (ALF). This study explored the potential molecular mechanism(s) of JDHY granules in promoting liver regeneration and preventing ALF. METHODS: Rat models of ALF were constructed through administration of D-galactosamine (D-GalN) (600 mg/kg) and lipopolysaccharides (LPS) (20 μg/kg). Rats were gavaged with JDHY granules, and serum and liver samples were collected at 12 h post-D-GalN/LPS administration. The degree of liver injury was evaluated through hepatic pathology and alanine/aspartate aminotransferase (ALT/AST) activity. miRNA chips were used to detect the miRNA expression profiles of rat models. Bioinformatics analysis was used to identify the biological processes and cell signaling pathways mediating the therapeutic effects of JDHY. Real-time PCR (RT-PCR) and western blotting were used to validate the data. RESULTS: JDHY granules could effectively decrease the levels of ALT and AST, relieve D-GalN/LPS-induced liver injury, and improve hepatic function. JDHY granules were found to regulate the expression of 20 miRNAs and 19 mRNAs, which influenced 21 biological processes and 9 signaling pathways. Upon analysis of the therapeutic mechanism(s) governing the effects of JDHY granules on liver regeneration, enhanced DNA replication and an improved cholesterol metabolic ratio were identified. JDHY granules were also found to increase the expression of MCM3, CDK4, and TC, confirming the involvement of these pathways. Moreover, JDHY granules were found to promote hepatocyte mitosis and inhibit the progression of ALF. CONCLUSION: JDHY granules protect against D-GalN/LPS-induced ALF in rats by promoting liver regeneration through enhanced DNA replication and an improved cholesterol metabolic ratio. |
format | Online Article Text |
id | pubmed-7787748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-77877482021-01-14 Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis Wang, Tingshuai Wang, Na Zhang, Rongzhen Huang, Shaodong Qiu, Hua Long, Fuli Wang, Minggang Mao, Dewen Evid Based Complement Alternat Med Research Article PURPOSE: Jie-Du-Hua-Yu (JDHY) granules are a traditional Chinese medicine with known therapeutic effects for the treatment of acute liver failure (ALF). This study explored the potential molecular mechanism(s) of JDHY granules in promoting liver regeneration and preventing ALF. METHODS: Rat models of ALF were constructed through administration of D-galactosamine (D-GalN) (600 mg/kg) and lipopolysaccharides (LPS) (20 μg/kg). Rats were gavaged with JDHY granules, and serum and liver samples were collected at 12 h post-D-GalN/LPS administration. The degree of liver injury was evaluated through hepatic pathology and alanine/aspartate aminotransferase (ALT/AST) activity. miRNA chips were used to detect the miRNA expression profiles of rat models. Bioinformatics analysis was used to identify the biological processes and cell signaling pathways mediating the therapeutic effects of JDHY. Real-time PCR (RT-PCR) and western blotting were used to validate the data. RESULTS: JDHY granules could effectively decrease the levels of ALT and AST, relieve D-GalN/LPS-induced liver injury, and improve hepatic function. JDHY granules were found to regulate the expression of 20 miRNAs and 19 mRNAs, which influenced 21 biological processes and 9 signaling pathways. Upon analysis of the therapeutic mechanism(s) governing the effects of JDHY granules on liver regeneration, enhanced DNA replication and an improved cholesterol metabolic ratio were identified. JDHY granules were also found to increase the expression of MCM3, CDK4, and TC, confirming the involvement of these pathways. Moreover, JDHY granules were found to promote hepatocyte mitosis and inhibit the progression of ALF. CONCLUSION: JDHY granules protect against D-GalN/LPS-induced ALF in rats by promoting liver regeneration through enhanced DNA replication and an improved cholesterol metabolic ratio. Hindawi 2020-12-30 /pmc/articles/PMC7787748/ /pubmed/33456491 http://dx.doi.org/10.1155/2020/8180959 Text en Copyright © 2020 Tingshuai Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Tingshuai Wang, Na Zhang, Rongzhen Huang, Shaodong Qiu, Hua Long, Fuli Wang, Minggang Mao, Dewen Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title | Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title_full | Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title_fullStr | Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title_full_unstemmed | Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title_short | Jie-Du-Hua-Yu Granules Promote Liver Regeneration in Rat Models of Acute Liver Failure: miRNA-mRNA Expression Analysis |
title_sort | jie-du-hua-yu granules promote liver regeneration in rat models of acute liver failure: mirna-mrna expression analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787748/ https://www.ncbi.nlm.nih.gov/pubmed/33456491 http://dx.doi.org/10.1155/2020/8180959 |
work_keys_str_mv | AT wangtingshuai jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT wangna jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT zhangrongzhen jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT huangshaodong jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT qiuhua jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT longfuli jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT wangminggang jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis AT maodewen jieduhuayugranulespromoteliverregenerationinratmodelsofacuteliverfailuremirnamrnaexpressionanalysis |