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YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway
Heart failure (HF) has been known as a global health problem, and cardiac remodeling plays an essential role in the development of HF. We hypothesized that YQWY decoction might exert a cardioprotective effect against myocardium inflammation, fibrosis, and apoptosis via activating the interleukin-10...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787750/ https://www.ncbi.nlm.nih.gov/pubmed/33456490 http://dx.doi.org/10.1155/2020/7532892 |
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author | Li, He Gong, Zhi-Jun He, Yun Huang, Jing-Jing Jiang, Yu-Ning Liu, Ya-Yang Zhu, Yao Jiang, Wei-Min |
author_facet | Li, He Gong, Zhi-Jun He, Yun Huang, Jing-Jing Jiang, Yu-Ning Liu, Ya-Yang Zhu, Yao Jiang, Wei-Min |
author_sort | Li, He |
collection | PubMed |
description | Heart failure (HF) has been known as a global health problem, and cardiac remodeling plays an essential role in the development of HF. We hypothesized that YQWY decoction might exert a cardioprotective effect against myocardium inflammation, fibrosis, and apoptosis via activating the interleukin-10 (IL-10)/Stat3 signaling pathway. To test this hypothesis, the HF model in rats was established by pressure overload through the minimally invasive transverse aortic constriction (MTAC). Echocardiography was performed to assess the left ventricular function of rats. Myocardial fibrosis in rats was observed by Masson and Picrosirius red staining, and the degree of myocardial apoptosis was detected via TUNEL staining. In addition, expression levels of IL-10, tumor necrosis factor-α (TNF-α), Stat3 (P-Stat3), P65 (P-P65), CD68, collagen I, TGF-β, CTGF, Bax, Bcl-2, cleaved caspase-3, and PARP in rat serum and myocardium samples were examined by ELISA, western blot, and immunohistochemistry, respectively. YQWY decoction treatment significantly improved left ventricular function in HF rats, especially in those of the high-dose group (LVEF%: 51.29 ± 5.876 vs. 66.02 ± 1.264, P < 0.01;, LVFS%: 27.75 ± 3.757 vs. 37.76 ± 1.137, P < 0.01). Furthermore, YQWY decoction markedly inhibited MTAC-induced myocardial fibrosis as evidenced by downregulated collagen I, TGF-β, and CTGF in myocardium and alleviated apoptosis (downregulated caspase-3 and PARP and increased Bcl-2/Bax ratio in cardiomyocytes). In addition, YQWY decoction decreased the level of the proinflammatory cytokine TNF-α in both circulating blood and myocardium and attenuated infiltration of inflammatory cells in heart tissue from HF rats. Most importantly, YQWY decoction suppressed MTAC-induced NF-κB activation and phosphorylated Stat3 by upregulating IL-10 in rat heart tissues. Our study showed that YQWY decoction could attenuate MTAC-induced myocardial inflammation, fibrosis, apoptosis, and reverse the impairment of cardiac function in rats by activating the IL-10/Stat3 signaling pathway and improving myocardium remodeling. Our findings suggested a therapeutic potential of YQWY decoction in HF. |
format | Online Article Text |
id | pubmed-7787750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-77877502021-01-14 YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway Li, He Gong, Zhi-Jun He, Yun Huang, Jing-Jing Jiang, Yu-Ning Liu, Ya-Yang Zhu, Yao Jiang, Wei-Min Evid Based Complement Alternat Med Research Article Heart failure (HF) has been known as a global health problem, and cardiac remodeling plays an essential role in the development of HF. We hypothesized that YQWY decoction might exert a cardioprotective effect against myocardium inflammation, fibrosis, and apoptosis via activating the interleukin-10 (IL-10)/Stat3 signaling pathway. To test this hypothesis, the HF model in rats was established by pressure overload through the minimally invasive transverse aortic constriction (MTAC). Echocardiography was performed to assess the left ventricular function of rats. Myocardial fibrosis in rats was observed by Masson and Picrosirius red staining, and the degree of myocardial apoptosis was detected via TUNEL staining. In addition, expression levels of IL-10, tumor necrosis factor-α (TNF-α), Stat3 (P-Stat3), P65 (P-P65), CD68, collagen I, TGF-β, CTGF, Bax, Bcl-2, cleaved caspase-3, and PARP in rat serum and myocardium samples were examined by ELISA, western blot, and immunohistochemistry, respectively. YQWY decoction treatment significantly improved left ventricular function in HF rats, especially in those of the high-dose group (LVEF%: 51.29 ± 5.876 vs. 66.02 ± 1.264, P < 0.01;, LVFS%: 27.75 ± 3.757 vs. 37.76 ± 1.137, P < 0.01). Furthermore, YQWY decoction markedly inhibited MTAC-induced myocardial fibrosis as evidenced by downregulated collagen I, TGF-β, and CTGF in myocardium and alleviated apoptosis (downregulated caspase-3 and PARP and increased Bcl-2/Bax ratio in cardiomyocytes). In addition, YQWY decoction decreased the level of the proinflammatory cytokine TNF-α in both circulating blood and myocardium and attenuated infiltration of inflammatory cells in heart tissue from HF rats. Most importantly, YQWY decoction suppressed MTAC-induced NF-κB activation and phosphorylated Stat3 by upregulating IL-10 in rat heart tissues. Our study showed that YQWY decoction could attenuate MTAC-induced myocardial inflammation, fibrosis, apoptosis, and reverse the impairment of cardiac function in rats by activating the IL-10/Stat3 signaling pathway and improving myocardium remodeling. Our findings suggested a therapeutic potential of YQWY decoction in HF. Hindawi 2020-12-14 /pmc/articles/PMC7787750/ /pubmed/33456490 http://dx.doi.org/10.1155/2020/7532892 Text en Copyright © 2020 He Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, He Gong, Zhi-Jun He, Yun Huang, Jing-Jing Jiang, Yu-Ning Liu, Ya-Yang Zhu, Yao Jiang, Wei-Min YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title | YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title_full | YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title_fullStr | YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title_full_unstemmed | YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title_short | YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling Pathway |
title_sort | yqwy decoction improves myocardial remodeling via activating the il-10/stat3 signaling pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787750/ https://www.ncbi.nlm.nih.gov/pubmed/33456490 http://dx.doi.org/10.1155/2020/7532892 |
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