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Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization
Macrophage-mediated inflammation compromises bone repair in diabetic patients. Electrical signaling cues are known to regulate macrophage functions. However, the biological effects of electrical microenvironment from charged biomaterials on the immune response for regulating osteogenesis under diabe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787955/ https://www.ncbi.nlm.nih.gov/pubmed/33474514 http://dx.doi.org/10.1016/j.bioactmat.2020.12.020 |
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author | Dai, Xiaohan Heng, Boon Chin Bai, Yunyang You, Fuping Sun, Xiaowen Li, Yiping Tang, Zhangui Xu, Mingming Zhang, Xuehui Deng, Xuliang |
author_facet | Dai, Xiaohan Heng, Boon Chin Bai, Yunyang You, Fuping Sun, Xiaowen Li, Yiping Tang, Zhangui Xu, Mingming Zhang, Xuehui Deng, Xuliang |
author_sort | Dai, Xiaohan |
collection | PubMed |
description | Macrophage-mediated inflammation compromises bone repair in diabetic patients. Electrical signaling cues are known to regulate macrophage functions. However, the biological effects of electrical microenvironment from charged biomaterials on the immune response for regulating osteogenesis under diabetic conditions remain to be elucidated. Herein the endogeneous electrical microenvironment of native bone tissue was recapitulated by fabricating a ferroelectric BaTiO(3)/poly (vinylidene fluoridetrifluoroethylene) (BTO/P(VDF-TrFE)) nanocomposite membrane. In vitro, the polarized BaTiO(3)/poly (vinylidene fluoridetrifluoroethylene) (BTO/P(VDF-TrFE)) nanocomposite membranes inhibited high glucose-induced M1-type inflammation, by effecting changes in cell morphology, M1 marker expression and pro-inflammatory cytokine secretion in macrophages. This led to enhanced osteogenic differentiation of human bone marrow mesenchymal stem cells (BM-MSCs). In vivo, the biomimetic electrical microenvironment recapitulated by the polarized nanocomposite membranes switched macrophage phenotype from the pro-inflammatory (M1) into the pro-healing (M2) phenotype, which in turn enhanced bone regeneration in rats with type 2 diabetes mellitus. Mechanistic studies revealed that the biomimetic electrical microenvironment attenuated pro-inflammatory M1 macrophage polarization under hyperglycemic conditions by suppressing expression of AKT2 and IRF5 within the PI3K-AKT signaling pathway, thereby inducing favorable osteo-immunomodulatory effects. Our study thus provides fundamental insights into the biological effects of restoring the electrical microenvironment conducive for osteogenesis under DM conditions, and offers an effective strategy to design functionalized biomaterials for bone regeneration therapy in diabetic patients. |
format | Online Article Text |
id | pubmed-7787955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-77879552021-01-19 Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization Dai, Xiaohan Heng, Boon Chin Bai, Yunyang You, Fuping Sun, Xiaowen Li, Yiping Tang, Zhangui Xu, Mingming Zhang, Xuehui Deng, Xuliang Bioact Mater Article Macrophage-mediated inflammation compromises bone repair in diabetic patients. Electrical signaling cues are known to regulate macrophage functions. However, the biological effects of electrical microenvironment from charged biomaterials on the immune response for regulating osteogenesis under diabetic conditions remain to be elucidated. Herein the endogeneous electrical microenvironment of native bone tissue was recapitulated by fabricating a ferroelectric BaTiO(3)/poly (vinylidene fluoridetrifluoroethylene) (BTO/P(VDF-TrFE)) nanocomposite membrane. In vitro, the polarized BaTiO(3)/poly (vinylidene fluoridetrifluoroethylene) (BTO/P(VDF-TrFE)) nanocomposite membranes inhibited high glucose-induced M1-type inflammation, by effecting changes in cell morphology, M1 marker expression and pro-inflammatory cytokine secretion in macrophages. This led to enhanced osteogenic differentiation of human bone marrow mesenchymal stem cells (BM-MSCs). In vivo, the biomimetic electrical microenvironment recapitulated by the polarized nanocomposite membranes switched macrophage phenotype from the pro-inflammatory (M1) into the pro-healing (M2) phenotype, which in turn enhanced bone regeneration in rats with type 2 diabetes mellitus. Mechanistic studies revealed that the biomimetic electrical microenvironment attenuated pro-inflammatory M1 macrophage polarization under hyperglycemic conditions by suppressing expression of AKT2 and IRF5 within the PI3K-AKT signaling pathway, thereby inducing favorable osteo-immunomodulatory effects. Our study thus provides fundamental insights into the biological effects of restoring the electrical microenvironment conducive for osteogenesis under DM conditions, and offers an effective strategy to design functionalized biomaterials for bone regeneration therapy in diabetic patients. KeAi Publishing 2020-12-31 /pmc/articles/PMC7787955/ /pubmed/33474514 http://dx.doi.org/10.1016/j.bioactmat.2020.12.020 Text en © 2020 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Dai, Xiaohan Heng, Boon Chin Bai, Yunyang You, Fuping Sun, Xiaowen Li, Yiping Tang, Zhangui Xu, Mingming Zhang, Xuehui Deng, Xuliang Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title_full | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title_fullStr | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title_full_unstemmed | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title_short | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
title_sort | restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787955/ https://www.ncbi.nlm.nih.gov/pubmed/33474514 http://dx.doi.org/10.1016/j.bioactmat.2020.12.020 |
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