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Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots

One major challenge in current microbubble (MB) and tissue plasminogen activator (tPA)-mediated sonothrombolysis techniques is effectively treating retracted blood clots, owing to the high density and low porosity of retracted clots. Nanodroplets (NDs) have the potential to enhance retracted clot ly...

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Autores principales: Goel, Leela, Wu, Huaiyu, Zhang, Bohua, Kim, Jinwook, Dayton, Paul A., Xu, Zhen, Jiang, Xiaoning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787976/
https://www.ncbi.nlm.nih.gov/pubmed/33456783
http://dx.doi.org/10.1038/s41378-020-00228-9
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author Goel, Leela
Wu, Huaiyu
Zhang, Bohua
Kim, Jinwook
Dayton, Paul A.
Xu, Zhen
Jiang, Xiaoning
author_facet Goel, Leela
Wu, Huaiyu
Zhang, Bohua
Kim, Jinwook
Dayton, Paul A.
Xu, Zhen
Jiang, Xiaoning
author_sort Goel, Leela
collection PubMed
description One major challenge in current microbubble (MB) and tissue plasminogen activator (tPA)-mediated sonothrombolysis techniques is effectively treating retracted blood clots, owing to the high density and low porosity of retracted clots. Nanodroplets (NDs) have the potential to enhance retracted clot lysis owing to their small size and ability to penetrate into retracted clots to enhance drug delivery. For the first time, we demonstrate that a sub-megahertz, forward-viewing intravascular (FVI) transducer can be used for ND-mediated sonothrombolysis, in vitro. In this study, we determined the minimum peak negative pressure to induce cavitation with low-boiling point phase change nanodroplets and clot lysis. We then compared nanodroplet mediated sonothrombolysis to MB and tPA mediate techniques. The clot lysis as a percent mass decrease in retracted clots was 9 ± 8%, 9 ± 5%, 16 ± 5%, 14 ± 9%, 17 ± 9%, 30 ± 8%, and 40 ± 9% for the control group, tPA alone, tPA + US, MB + US, MB + tPA + US, ND + US, and ND + tPA + US groups, respectively. In retracted blood clots, combined ND- and tPA-mediated sonothrombolysis was able to significantly enhance retracted clot lysis compared with traditional MB and tPA-mediated sonothrombolysis techniques. Combined nanodroplet with tPA-mediated sonothrombolysis may provide a feasible strategy for safely treating retracted clots.
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spelling pubmed-77879762021-01-14 Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots Goel, Leela Wu, Huaiyu Zhang, Bohua Kim, Jinwook Dayton, Paul A. Xu, Zhen Jiang, Xiaoning Microsyst Nanoeng Article One major challenge in current microbubble (MB) and tissue plasminogen activator (tPA)-mediated sonothrombolysis techniques is effectively treating retracted blood clots, owing to the high density and low porosity of retracted clots. Nanodroplets (NDs) have the potential to enhance retracted clot lysis owing to their small size and ability to penetrate into retracted clots to enhance drug delivery. For the first time, we demonstrate that a sub-megahertz, forward-viewing intravascular (FVI) transducer can be used for ND-mediated sonothrombolysis, in vitro. In this study, we determined the minimum peak negative pressure to induce cavitation with low-boiling point phase change nanodroplets and clot lysis. We then compared nanodroplet mediated sonothrombolysis to MB and tPA mediate techniques. The clot lysis as a percent mass decrease in retracted clots was 9 ± 8%, 9 ± 5%, 16 ± 5%, 14 ± 9%, 17 ± 9%, 30 ± 8%, and 40 ± 9% for the control group, tPA alone, tPA + US, MB + US, MB + tPA + US, ND + US, and ND + tPA + US groups, respectively. In retracted blood clots, combined ND- and tPA-mediated sonothrombolysis was able to significantly enhance retracted clot lysis compared with traditional MB and tPA-mediated sonothrombolysis techniques. Combined nanodroplet with tPA-mediated sonothrombolysis may provide a feasible strategy for safely treating retracted clots. Nature Publishing Group UK 2021-01-06 /pmc/articles/PMC7787976/ /pubmed/33456783 http://dx.doi.org/10.1038/s41378-020-00228-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Goel, Leela
Wu, Huaiyu
Zhang, Bohua
Kim, Jinwook
Dayton, Paul A.
Xu, Zhen
Jiang, Xiaoning
Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title_full Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title_fullStr Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title_full_unstemmed Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title_short Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
title_sort nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787976/
https://www.ncbi.nlm.nih.gov/pubmed/33456783
http://dx.doi.org/10.1038/s41378-020-00228-9
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