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Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation
Alveolar type II cells (AT2) are stem cells of lung epithelium. To study these cells, we developed protocols to isolate primary AT2 and test the effects of potential niche factors in regulating the AT2 progenitor functions. AT2 freshly isolated from mouse lungs are grown in 2D or 3D culture. AT2 are...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788236/ https://www.ncbi.nlm.nih.gov/pubmed/33437966 http://dx.doi.org/10.1016/j.xpro.2020.100241 |