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Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources

Sepsis, one of the most fatal diseases in the world, is known to culminate in multiple organ failure due to an uncontrolled inflammatory response. Hence, the use of animal models in sepsis research is very important to study complex immune responses. The current study was undertaken to compare comme...

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Autores principales: Park, Ji Won, Lee, Su Jin, Kim, Ji Eun, Kang, Mi Ju, Bae, Su Ji, Choi, Yun Ju, Gong, Jeong Eun, Kim, Kil Soo, Jung, Young-Suk, Cho, Joon-Yong, Choi, Yeon Shik, Hwang, Dae Youn, Song, Hyun Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788770/
https://www.ncbi.nlm.nih.gov/pubmed/33407886
http://dx.doi.org/10.1186/s42826-020-00079-5
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author Park, Ji Won
Lee, Su Jin
Kim, Ji Eun
Kang, Mi Ju
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Kim, Kil Soo
Jung, Young-Suk
Cho, Joon-Yong
Choi, Yeon Shik
Hwang, Dae Youn
Song, Hyun Keun
author_facet Park, Ji Won
Lee, Su Jin
Kim, Ji Eun
Kang, Mi Ju
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Kim, Kil Soo
Jung, Young-Suk
Cho, Joon-Yong
Choi, Yeon Shik
Hwang, Dae Youn
Song, Hyun Keun
author_sort Park, Ji Won
collection PubMed
description Sepsis, one of the most fatal diseases in the world, is known to culminate in multiple organ failure due to an uncontrolled inflammatory response. Hence, the use of animal models in sepsis research is very important to study complex immune responses. The current study was undertaken to compare commercial stocks with KFDA stocks of DBA/2 mice as an animal model for sepsis study. To compare responses of DBA/2 mice to lipopolysaccharides (LPS)-induced sepsis, we measured altered characteristics of various factors associated with sepsis, including survival curves, organ failure and inflammatory response, in DBA/2Korl stock and two commercial stocks (DBA/2A and DBA/2B). Survival rates after LPS exposure were similar for DBA/2Korl and DBA/2B; however, for times over 20 h, survival rates were reduced and concentration dependent in DBA/2A. In order to evaluate multiple organ failure caused by sepsis, H&E stains were evaluated for liver and spleen tissues obtained in the early (2 h) and later (20 h) stages after exposure to LPS; no significant differences were observed between the three stocks. mRNA and protein levels of proinflammatory cytokines were assessed for evaluating inflammatory reactions, and were found to increase in a dose-dependent manner in most DBA/2 mice after LPS treatment. However, no changes were observed in the mRNA levels of proinflammatory cytokines at 20 h after LPS exposure in the DBA/2A stock. The induction of inflammation-mediated factors by LPS exposure did not induce alterations in the mRNA levels of COX-2 and iNOS in all three DBA/2 stocks. Our results indicate that response of DBA/2Korl to LPS-induced sepsis is similar to the two commercial DBA/2 stocks, thus representing its potential as a useful biological resource established in Korea.
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spelling pubmed-77887702021-01-07 Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources Park, Ji Won Lee, Su Jin Kim, Ji Eun Kang, Mi Ju Bae, Su Ji Choi, Yun Ju Gong, Jeong Eun Kim, Kil Soo Jung, Young-Suk Cho, Joon-Yong Choi, Yeon Shik Hwang, Dae Youn Song, Hyun Keun Lab Anim Res Research Sepsis, one of the most fatal diseases in the world, is known to culminate in multiple organ failure due to an uncontrolled inflammatory response. Hence, the use of animal models in sepsis research is very important to study complex immune responses. The current study was undertaken to compare commercial stocks with KFDA stocks of DBA/2 mice as an animal model for sepsis study. To compare responses of DBA/2 mice to lipopolysaccharides (LPS)-induced sepsis, we measured altered characteristics of various factors associated with sepsis, including survival curves, organ failure and inflammatory response, in DBA/2Korl stock and two commercial stocks (DBA/2A and DBA/2B). Survival rates after LPS exposure were similar for DBA/2Korl and DBA/2B; however, for times over 20 h, survival rates were reduced and concentration dependent in DBA/2A. In order to evaluate multiple organ failure caused by sepsis, H&E stains were evaluated for liver and spleen tissues obtained in the early (2 h) and later (20 h) stages after exposure to LPS; no significant differences were observed between the three stocks. mRNA and protein levels of proinflammatory cytokines were assessed for evaluating inflammatory reactions, and were found to increase in a dose-dependent manner in most DBA/2 mice after LPS treatment. However, no changes were observed in the mRNA levels of proinflammatory cytokines at 20 h after LPS exposure in the DBA/2A stock. The induction of inflammation-mediated factors by LPS exposure did not induce alterations in the mRNA levels of COX-2 and iNOS in all three DBA/2 stocks. Our results indicate that response of DBA/2Korl to LPS-induced sepsis is similar to the two commercial DBA/2 stocks, thus representing its potential as a useful biological resource established in Korea. BioMed Central 2021-01-07 /pmc/articles/PMC7788770/ /pubmed/33407886 http://dx.doi.org/10.1186/s42826-020-00079-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Park, Ji Won
Lee, Su Jin
Kim, Ji Eun
Kang, Mi Ju
Bae, Su Ji
Choi, Yun Ju
Gong, Jeong Eun
Kim, Kil Soo
Jung, Young-Suk
Cho, Joon-Yong
Choi, Yeon Shik
Hwang, Dae Youn
Song, Hyun Keun
Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title_full Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title_fullStr Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title_full_unstemmed Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title_short Comparison of response to LPS-induced sepsis in three DBA/2 stocks derived from different sources
title_sort comparison of response to lps-induced sepsis in three dba/2 stocks derived from different sources
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788770/
https://www.ncbi.nlm.nih.gov/pubmed/33407886
http://dx.doi.org/10.1186/s42826-020-00079-5
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