Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

OBJECTIVE: Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for gene edit...

Descripción completa

Detalles Bibliográficos
Autores principales: Le, Quynh Anh, Tanihara, Fuminori, Wittayarat, Manita, Namula, Zhao, Sato, Yoko, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788904/
https://www.ncbi.nlm.nih.gov/pubmed/33407863
http://dx.doi.org/10.1186/s13104-020-05412-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788904/
https://www.ncbi.nlm.nih.gov/pubmed/33407863
http://dx.doi.org/10.1186/s13104-020-05412-8

Ejemplares similares