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Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
OBJECTIVE: Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for gene edit...
Autores principales: | Le, Quynh Anh, Tanihara, Fuminori, Wittayarat, Manita, Namula, Zhao, Sato, Yoko, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Otoi, Takeshige |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788904/ https://www.ncbi.nlm.nih.gov/pubmed/33407863 http://dx.doi.org/10.1186/s13104-020-05412-8 |
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