The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats

PURPOSE: To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. METHOD: 128 three-week-old weaned rats underwent high-fat diet and exercise (60–70% VO(2max), 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Junpeng, Xu, Rui, Li, Yafei, Zhou, Qishu, Song, Ge, Deng, Yimin, Yan, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788936/
https://www.ncbi.nlm.nih.gov/pubmed/33407658
http://dx.doi.org/10.1186/s12986-020-00517-0
_version_ 1783633132344311808
author Feng, Junpeng
Xu, Rui
Li, Yafei
Zhou, Qishu
Song, Ge
Deng, Yimin
Yan, Yi
author_facet Feng, Junpeng
Xu, Rui
Li, Yafei
Zhou, Qishu
Song, Ge
Deng, Yimin
Yan, Yi
author_sort Feng, Junpeng
collection PubMed
description PURPOSE: To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. METHOD: 128 three-week-old weaned rats underwent high-fat diet and exercise (60–70% VO(2max), 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting. RESULT: (1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60–70% VO(2)max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet. CONCLUSION: The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60–70% VO(2)max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.
format Online
Article
Text
id pubmed-7788936
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-77889362021-01-07 The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats Feng, Junpeng Xu, Rui Li, Yafei Zhou, Qishu Song, Ge Deng, Yimin Yan, Yi Nutr Metab (Lond) Research PURPOSE: To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. METHOD: 128 three-week-old weaned rats underwent high-fat diet and exercise (60–70% VO(2max), 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting. RESULT: (1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60–70% VO(2)max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet. CONCLUSION: The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60–70% VO(2)max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders. BioMed Central 2021-01-06 /pmc/articles/PMC7788936/ /pubmed/33407658 http://dx.doi.org/10.1186/s12986-020-00517-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Feng, Junpeng
Xu, Rui
Li, Yafei
Zhou, Qishu
Song, Ge
Deng, Yimin
Yan, Yi
The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title_full The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title_fullStr The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title_full_unstemmed The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title_short The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats
title_sort effect of high-fat diet and exercise on kiss-1/gpr54 expression in testis of growing rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788936/
https://www.ncbi.nlm.nih.gov/pubmed/33407658
http://dx.doi.org/10.1186/s12986-020-00517-0
work_keys_str_mv AT fengjunpeng theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT xurui theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT liyafei theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT zhouqishu theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT songge theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT dengyimin theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT yanyi theeffectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT fengjunpeng effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT xurui effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT liyafei effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT zhouqishu effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT songge effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT dengyimin effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats
AT yanyi effectofhighfatdietandexerciseonkiss1gpr54expressionintestisofgrowingrats

Ejemplares similares