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Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit

BACKGROUND: Caffeic acid O-methyltransferases (COMTs) play an important role in the diversification of natural products, especially in the phenylalanine metabolic pathway of plant. The content of COMT genes in blueberry and relationship between their expression patterns and the lignin content during...

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Autores principales: Liu, Yushan, Wang, Yizhou, Pei, Jiabo, Li, Yadong, Sun, Haiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789564/
https://www.ncbi.nlm.nih.gov/pubmed/33407129
http://dx.doi.org/10.1186/s12870-020-02767-9
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author Liu, Yushan
Wang, Yizhou
Pei, Jiabo
Li, Yadong
Sun, Haiyue
author_facet Liu, Yushan
Wang, Yizhou
Pei, Jiabo
Li, Yadong
Sun, Haiyue
author_sort Liu, Yushan
collection PubMed
description BACKGROUND: Caffeic acid O-methyltransferases (COMTs) play an important role in the diversification of natural products, especially in the phenylalanine metabolic pathway of plant. The content of COMT genes in blueberry and relationship between their expression patterns and the lignin content during fruit development have not clearly investigated by now. RESULTS: Ninety-two VcCOMTs were identified in Vaccinium corymbosum. According to phylogenetic analyses, the 92 VcCOMTs were divided into 2 groups. The gene structure and conserved motifs within groups were similar which supported the reliability of the phylogenetic structure groupings. Dispersed duplication (DSD) and whole-genome duplication (WGD) were determined to be the major forces in VcCOMTs evolution. The results showed that the results of qRT-PCR and lignin content for 22 VcCOMTs, VcCOMT40 and VcCOMT92 were related to lignin content at different stages of fruit development of blueberry. CONCLUSION: We identified COMT gene family in blueberry, and performed comparative analyses of the phylogenetic relationships in the 15 species of land plant, and gene duplication patterns of COMT genes in 5 of the 15 species. We found 2 VcCOMTs were highly expressed and their relative contents were similar to the variation trend of lignin content during the development of blueberry fruit. These results provide a clue for further study on the roles of VcCOMTs in the development of blueberry fruit and could promisingly be foundations for breeding blueberry clutivals with higher fruit firmness and longer shelf life. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-020-02767-9.
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spelling pubmed-77895642021-01-07 Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit Liu, Yushan Wang, Yizhou Pei, Jiabo Li, Yadong Sun, Haiyue BMC Plant Biol Research Article BACKGROUND: Caffeic acid O-methyltransferases (COMTs) play an important role in the diversification of natural products, especially in the phenylalanine metabolic pathway of plant. The content of COMT genes in blueberry and relationship between their expression patterns and the lignin content during fruit development have not clearly investigated by now. RESULTS: Ninety-two VcCOMTs were identified in Vaccinium corymbosum. According to phylogenetic analyses, the 92 VcCOMTs were divided into 2 groups. The gene structure and conserved motifs within groups were similar which supported the reliability of the phylogenetic structure groupings. Dispersed duplication (DSD) and whole-genome duplication (WGD) were determined to be the major forces in VcCOMTs evolution. The results showed that the results of qRT-PCR and lignin content for 22 VcCOMTs, VcCOMT40 and VcCOMT92 were related to lignin content at different stages of fruit development of blueberry. CONCLUSION: We identified COMT gene family in blueberry, and performed comparative analyses of the phylogenetic relationships in the 15 species of land plant, and gene duplication patterns of COMT genes in 5 of the 15 species. We found 2 VcCOMTs were highly expressed and their relative contents were similar to the variation trend of lignin content during the development of blueberry fruit. These results provide a clue for further study on the roles of VcCOMTs in the development of blueberry fruit and could promisingly be foundations for breeding blueberry clutivals with higher fruit firmness and longer shelf life. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-020-02767-9. BioMed Central 2021-01-06 /pmc/articles/PMC7789564/ /pubmed/33407129 http://dx.doi.org/10.1186/s12870-020-02767-9 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Liu, Yushan
Wang, Yizhou
Pei, Jiabo
Li, Yadong
Sun, Haiyue
Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title_full Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title_fullStr Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title_full_unstemmed Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title_short Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
title_sort genome-wide identification and characterization of comt gene family during the development of blueberry fruit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789564/
https://www.ncbi.nlm.nih.gov/pubmed/33407129
http://dx.doi.org/10.1186/s12870-020-02767-9
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