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Lifestyle modifications result in alterations in the gut microbiota in obese children

BACKGROUND: The association between the gut microbiota and pediatric obesity was analyzed in a cross-sectional study. A prospective study of obese children was conducted to assess the gut microbial alterations after a weight change. We collected fecal samples from obese children before and after a 2...

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Autor principal: Cho, Ky Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789654/
https://www.ncbi.nlm.nih.gov/pubmed/33407104
http://dx.doi.org/10.1186/s12866-020-02002-3
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author Cho, Ky Young
author_facet Cho, Ky Young
author_sort Cho, Ky Young
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description BACKGROUND: The association between the gut microbiota and pediatric obesity was analyzed in a cross-sectional study. A prospective study of obese children was conducted to assess the gut microbial alterations after a weight change. We collected fecal samples from obese children before and after a 2-month weight reduction program that consisted of individual counseling for nutritional education and physical activity, and we performed 16S rRNA gene amplicon sequencing using an Illumina MiSeq platform. RESULTS: Thirty-six participants, aged 7 to 18 years, were classified into the fat loss (n = 17) and the fat gain (n = 19) groups according to the change in total body fat (%) after the intervention. The baseline analysis of the gut microbiota in the preintervention stages showed dysbiotic features of both groups compared with those of normal-weight children. In the fat loss group, significantly decreased proportions of Bacteroidetes phylum, Bacteroidia class, Bacteroidales order, Bacteroidaceae family, and Bacteroides genus, along with increased proportions of Firmicutes phylum, Clostridia class, and Clostridiales order, were observed after intervention. The microbial richness was significantly reduced, without a change in beta diversity in the fat loss group. The fat gain group showed significantly deceased proportions of Firmicutes phylum, Clostridia class, Clostridiales order, Lachnospiraceae family, and Eubacterium hallii group genus, without a change in diversity after the intervention. According to the functional metabolic analysis by the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2, the “Nitrate Reduction VI” and “Aspartate Superpathway” pathways were predicted to increase significantly in the fat loss group. The cooccurring networks of genera were constructed and showed the different microbes that drove the changes between the pre- and postintervention stages in the fat loss and fat gain groups. CONCLUSIONS: This study demonstrated that lifestyle modifications can impact the composition, richness, and predicted functional profiles of the gut microbiota in obese children after weight changes. TRIAL REGISTRATION: ClinicalTrials.govNCT03812497, registration date January 23, 2019, retrospectively registered. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12866-020-02002-3.
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spelling pubmed-77896542021-01-07 Lifestyle modifications result in alterations in the gut microbiota in obese children Cho, Ky Young BMC Microbiol Research Article BACKGROUND: The association between the gut microbiota and pediatric obesity was analyzed in a cross-sectional study. A prospective study of obese children was conducted to assess the gut microbial alterations after a weight change. We collected fecal samples from obese children before and after a 2-month weight reduction program that consisted of individual counseling for nutritional education and physical activity, and we performed 16S rRNA gene amplicon sequencing using an Illumina MiSeq platform. RESULTS: Thirty-six participants, aged 7 to 18 years, were classified into the fat loss (n = 17) and the fat gain (n = 19) groups according to the change in total body fat (%) after the intervention. The baseline analysis of the gut microbiota in the preintervention stages showed dysbiotic features of both groups compared with those of normal-weight children. In the fat loss group, significantly decreased proportions of Bacteroidetes phylum, Bacteroidia class, Bacteroidales order, Bacteroidaceae family, and Bacteroides genus, along with increased proportions of Firmicutes phylum, Clostridia class, and Clostridiales order, were observed after intervention. The microbial richness was significantly reduced, without a change in beta diversity in the fat loss group. The fat gain group showed significantly deceased proportions of Firmicutes phylum, Clostridia class, Clostridiales order, Lachnospiraceae family, and Eubacterium hallii group genus, without a change in diversity after the intervention. According to the functional metabolic analysis by the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2, the “Nitrate Reduction VI” and “Aspartate Superpathway” pathways were predicted to increase significantly in the fat loss group. The cooccurring networks of genera were constructed and showed the different microbes that drove the changes between the pre- and postintervention stages in the fat loss and fat gain groups. CONCLUSIONS: This study demonstrated that lifestyle modifications can impact the composition, richness, and predicted functional profiles of the gut microbiota in obese children after weight changes. TRIAL REGISTRATION: ClinicalTrials.govNCT03812497, registration date January 23, 2019, retrospectively registered. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12866-020-02002-3. BioMed Central 2021-01-06 /pmc/articles/PMC7789654/ /pubmed/33407104 http://dx.doi.org/10.1186/s12866-020-02002-3 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Cho, Ky Young
Lifestyle modifications result in alterations in the gut microbiota in obese children
title Lifestyle modifications result in alterations in the gut microbiota in obese children
title_full Lifestyle modifications result in alterations in the gut microbiota in obese children
title_fullStr Lifestyle modifications result in alterations in the gut microbiota in obese children
title_full_unstemmed Lifestyle modifications result in alterations in the gut microbiota in obese children
title_short Lifestyle modifications result in alterations in the gut microbiota in obese children
title_sort lifestyle modifications result in alterations in the gut microbiota in obese children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789654/
https://www.ncbi.nlm.nih.gov/pubmed/33407104
http://dx.doi.org/10.1186/s12866-020-02002-3
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