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Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis

The initiation of atopic dermatitis (AD) typically happens very early in life, but most of our understanding of AD is derived from studies on AD patients in adult. The aim of the present study was to identify gene signature speficic to pediatric AD comapred with adult AD. The gene expression profile...

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Autores principales: Wang, Tianyi, Zhang, Bingxin, Li, Danhui, Qi, Xiaoli, Zhang, Chijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789805/
https://www.ncbi.nlm.nih.gov/pubmed/33289509
http://dx.doi.org/10.1042/BSR20193517
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author Wang, Tianyi
Zhang, Bingxin
Li, Danhui
Qi, Xiaoli
Zhang, Chijin
author_facet Wang, Tianyi
Zhang, Bingxin
Li, Danhui
Qi, Xiaoli
Zhang, Chijin
author_sort Wang, Tianyi
collection PubMed
description The initiation of atopic dermatitis (AD) typically happens very early in life, but most of our understanding of AD is derived from studies on AD patients in adult. The aim of the present study was to identify gene signature speficic to pediatric AD comapred with adult AD. The gene expression profiles of four datasets (GSE32924, GSE36842, GSE58558, and GSE107361) were downloaded from the GEO database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and protein–protein interaction (PPI) network was constructed by Cytoscape software. Total 654 differentially expressed genes (DEGs) (394 up-regulated and 260 down-regulated) were identified in pediatric AD samples with adult AD samples as control. The up-regulated DEGs were significantly enriched in the migration and chemotaxis of granulocyte and neutrophil, while down-regulated DEGs were significantly enriched in biological adhesion. KEGG pathway analysis showed that up-regulated DEGs participated in chemokine signaling pathway while down-regulated DEGs participated in adherens junction, focal adhesion, and regulation of actin cytoskeleton. The top 10 hub genes GAPDH, EGFR, ACTB, ESR1, CDK1, CXCL8, CD44, KRAS, PTGS2, and SMC3 were involved in chemokine signaling pathway, cytokine–cytokine receptor interaction, interleukin-17 signaling pathway, and regulation of actin cytoskeleton. In conclusion, we identified DEGs and hub genes involved in pediatric AD, which might be used as therapeutic targets and diagnostic biomarkers for pediatric AD.
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spelling pubmed-77898052021-01-13 Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis Wang, Tianyi Zhang, Bingxin Li, Danhui Qi, Xiaoli Zhang, Chijin Biosci Rep Bioinformatics The initiation of atopic dermatitis (AD) typically happens very early in life, but most of our understanding of AD is derived from studies on AD patients in adult. The aim of the present study was to identify gene signature speficic to pediatric AD comapred with adult AD. The gene expression profiles of four datasets (GSE32924, GSE36842, GSE58558, and GSE107361) were downloaded from the GEO database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and protein–protein interaction (PPI) network was constructed by Cytoscape software. Total 654 differentially expressed genes (DEGs) (394 up-regulated and 260 down-regulated) were identified in pediatric AD samples with adult AD samples as control. The up-regulated DEGs were significantly enriched in the migration and chemotaxis of granulocyte and neutrophil, while down-regulated DEGs were significantly enriched in biological adhesion. KEGG pathway analysis showed that up-regulated DEGs participated in chemokine signaling pathway while down-regulated DEGs participated in adherens junction, focal adhesion, and regulation of actin cytoskeleton. The top 10 hub genes GAPDH, EGFR, ACTB, ESR1, CDK1, CXCL8, CD44, KRAS, PTGS2, and SMC3 were involved in chemokine signaling pathway, cytokine–cytokine receptor interaction, interleukin-17 signaling pathway, and regulation of actin cytoskeleton. In conclusion, we identified DEGs and hub genes involved in pediatric AD, which might be used as therapeutic targets and diagnostic biomarkers for pediatric AD. Portland Press Ltd. 2021-01-06 /pmc/articles/PMC7789805/ /pubmed/33289509 http://dx.doi.org/10.1042/BSR20193517 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bioinformatics
Wang, Tianyi
Zhang, Bingxin
Li, Danhui
Qi, Xiaoli
Zhang, Chijin
Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title_full Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title_fullStr Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title_full_unstemmed Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title_short Bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
title_sort bioinformatic analysis of key pathways and genes involved in pediatric atopic dermatitis
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789805/
https://www.ncbi.nlm.nih.gov/pubmed/33289509
http://dx.doi.org/10.1042/BSR20193517
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