Cargando…

Soluble expression and purification of Bluetongue Virus Type 1 (BTV1) structure protein VP2 in Escherichia coli and its immunogenicity in mice

BACKGROUND: The VP2 on the surface of the virus particle is the main structural protein of BTV, which can induce the host to produce neutralizing antibodies and play an important role in the antiviral immunity process. This study aimed to obtain the soluble VP2 and analyze its immunogenicity. METHOD...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Aiping, Yin, Jiajia, Zhou, Jingming, Ma, Hongfang, Chen, Yumei, Liu, Hongliang, Qi, Yanhua, Liang, Chao, Liu, Yankai, Li, Jinge, Zhang, Gaiping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	PeerJ Inc. 2021
Materias:	Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789859/ https://www.ncbi.nlm.nih.gov/pubmed/33505791 http://dx.doi.org/10.7717/peerj.10543

Ejemplares similares

Establishment of an Immunological Method for Detection of Bluetongue Virus by Fluorescence-Linked Immunosorbent Assay
por: Yin, Jiajia, et al.
Publicado: (2022)

Evaluation of Bluetongue Virus (BTV) Antibodies for the Immunohistochemical Detection of BTV and Other Orbiviruses
por: Lean, Fabian Z. X., et al.
Publicado: (2020)

Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV
por: Matsuo, Eiko, et al.
Publicado: (2014)

An Equine Herpesvirus Type 1 (EHV-1) Expressing VP2 and VP5 of Serotype 8 Bluetongue Virus (BTV-8) Induces Protection in a Murine Infection Model
por: Ma, Guanggang, et al.
Publicado: (2012)

Attenuation of Bluetongue Virus (BTV) in an in ovo Model Is Related to the Changes of Viral Genetic Diversity of Cell-Culture Passaged BTV
por: Lean, Fabian Z. X., et al.
Publicado: (2019)

Full-Genome Sequence of Bluetongue Virus Serotype 1 (BTV-1) Strain Y863, the First BTV-1 Isolate of Eastern Origin Found in China
por: Zhu, Jianbo, et al.
Publicado: (2013)

Assessment of cross-protection induced by a bluetongue virus (BTV) serotype 8 vaccine towards other BTV serotypes in experimental conditions
por: Martinelle, Ludovic, et al.
Publicado: (2018)

Presence of Antibodies against Bluetongue Virus (BTV) in Sheep 5 to 7.5 Years after Vaccination with Inactivated BTV-8 Vaccines
por: Hilke, Johanna, et al.
Publicado: (2019)

Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge
por: Moreno, Sandra, et al.
Publicado: (2020)

Serological survey of SARS-CoV-2 in companion animals in China
por: Wang, Aiping, et al.
Publicado: (2022)

Serological Investigations of Bluetongue Virus (BTV) among Sheep and Goats in Kassala State, Eastern Sudan
por: Elmahi, Molhima M., et al.
Publicado: (2020)

Expression of extracellular domain of ASFV CD2v protein in mammalian cells and identification of B cell epitopes
por: Liu, Hongliang, et al.
Publicado: (2022)

Diagnostic Reliability of Different RT-PCR Protocols for the Detection of Bluetongue Virus Serotype 14 (BTV-14)
por: Orłowska, Anna, et al.
Publicado: (2017)

Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV)
por: van Rijn, Piet A., et al.
Publicado: (2016)

Uncovering the Underlying Mechanisms Blocking Replication of Bluetongue Virus Serotype 26 (BTV-26) in Culicoides Cells
por: Monsion, Baptiste, et al.
Publicado: (2023)

Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus
por: Jia, Rui, et al.
Publicado: (2021)

A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein
por: Li, Zehui, et al.
Publicado: (2022)

A Dynamic Spatio-Temporal Model to Investigate the Effect of Cattle Movements on the Spread of Bluetongue BTV-8 in Belgium
por: Ensoy, Chellafe, et al.
Publicado: (2013)

Understanding Spatio-Temporal Variability in the Reproduction Ratio of the Bluetongue (BTV-1) Epidemic in Southern Spain (Andalusia) in 2007 Using Epidemic Trees
por: Napp, S., et al.
Publicado: (2016)

Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer
por: Haegeman, Andy, et al.
Publicado: (2019)

Electrochemical immunosensor nanoarchitectonics with the Ag-rGO nanocomposites for the detection of receptor-binding domain of SARS-CoV-2 spike protein
por: Wang, Aiping, et al.
Publicado: (2022)

Ovine and murine T cell epitopes from the non-structural protein 1 (NS1) of bluetongue virus serotype 8 (BTV-8) are shared among viral serotypes
por: Rojas, José M, et al.
Publicado: (2014)

Development of an Indirect ELISA Kit for Rapid Detection of Varicella-Zoster Virus Antibody by Glycoprotein E
por: Niu, Yan, et al.
Publicado: (2022)

Implications of a conserved region of bluetongue virus protein VP2 in cross-neutralisation of bluetongue virus serotypes
por: Jyothi, Shiva J., et al.
Publicado: (2020)

Production and Easy One-Step Purification of Bluetongue Recombinant VP7 from Infected Sf9 Supernatant for an Immunoenzymatic Assay (ELISA)
por: Ulisse, S., et al.
Publicado: (2020)

Transplacental transmission of field and rescued strains of BTV-2 and BTV-8 in experimentally infected sheep
por: Rasmussen, Lasse Dam, et al.
Publicado: (2013)

Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2
por: Jiang, Min, et al.
Publicado: (2023)

Complete Genome Sequences of Five Bluetongue Virus (BTV) Vaccine Strains from a Commercial Live Attenuated Vaccine, a BTV-4 Field Strain from South Africa, and a Reassortant Strain Isolated from Experimentally Vaccinated Cattle
por: van den Bergh, Carien, et al.
Publicado: (2016)

Bluetongue Virus VP1 Polymerase Activity In Vitro: Template Dependency, Dinucleotide Priming and Cap Dependency
por: Matsuo, Eiko, et al.
Publicado: (2011)

Rapid Visual Detection of Hepatitis C Virus Using Reverse Transcription Recombinase-Aided Amplification–Lateral Flow Dipstick
por: Wang, Haili, et al.
Publicado: (2022)

Simulation and Measurement Report for the SPS BTV
por: Farricker, Aaron, et al.
Publicado: (2020)

The Interaction of Bluetongue Virus VP6 and Genomic RNA Is Essential for Genome Packaging
por: Sung, Po-Yu, et al.
Publicado: (2019)

Screening and identification of B cell epitope of the nucleocapsid protein in SARS-CoV-2 using the monoclonal antibodies
por: Tian, Yuanyuan, et al.
Publicado: (2022)

Identification of a mimotope of an infectious bronchitis virus S1 protein
por: Zhou, Jingming, et al.
Publicado: (2021)

Bluetongue Viruses Based on Modified-Live Vaccine Serotype 6 with Exchanged Outer Shell Proteins Confer Full Protection in Sheep against Virulent BTV8
por: van Gennip, René G. P., et al.
Publicado: (2012)

Beam television monitor (BTV) as profile detector
por: Ferioli, G, et al.
Publicado: (1981)

Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
por: Rojas, José Manuel, et al.
Publicado: (2021)

Comparative Evaluation of T-Cell Immune Response to BTV Infection in Sheep Vaccinated with Pentavalent BTV Vaccine When Compared to Un-Vaccinated Animals
por: Bitew, Molalegne, et al.
Publicado: (2019)

Sialic Acid Binding Sites in VP2 of Bluetongue Virus and Their Use during Virus Entry
por: Wu, Weining, et al.
Publicado: (2022)

Interferon-stimulated gene 15 facilitates BTV replication through interacting with the NS1 protein
por: Kang, Di, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_7jfdd6ov4qsesk5pnqd2p91qu9