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The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens

Mycobacterial pathogens pose a sustained threat to human health. There is a critical need for new diagnostics, therapeutics, and vaccines targeting both tuberculous and nontuberculous mycobacterial species. Understanding the basic mechanisms used by diverse mycobacterial species to cause disease wil...

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Autores principales: Nicholson, Kathleen R., Mousseau, C. Bruce, Champion, Matthew M., Champion, Patricia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790235/
https://www.ncbi.nlm.nih.gov/pubmed/33411813
http://dx.doi.org/10.1371/journal.ppat.1009124
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author Nicholson, Kathleen R.
Mousseau, C. Bruce
Champion, Matthew M.
Champion, Patricia A.
author_facet Nicholson, Kathleen R.
Mousseau, C. Bruce
Champion, Matthew M.
Champion, Patricia A.
author_sort Nicholson, Kathleen R.
collection PubMed
description Mycobacterial pathogens pose a sustained threat to human health. There is a critical need for new diagnostics, therapeutics, and vaccines targeting both tuberculous and nontuberculous mycobacterial species. Understanding the basic mechanisms used by diverse mycobacterial species to cause disease will facilitate efforts to design new approaches toward detection, treatment, and prevention of mycobacterial disease. Molecular, genetic, and biochemical approaches have been widely employed to define fundamental aspects of mycobacterial physiology and virulence. The recent expansion of genetic tools in mycobacteria has further increased the accessibility of forward genetic approaches. Proteomics has also emerged as a powerful approach to further our understanding of diverse mycobacterial species. Detection of large numbers of proteins and their modifications from complex mixtures of mycobacterial proteins is now routine, with efforts of quantification of these datasets becoming more robust. In this review, we discuss the “genetic proteome,” how the power of genetics, molecular biology, and biochemistry informs and amplifies the quality of subsequent analytical approaches and maximizes the potential of hypothesis-driven mycobacterial research. Published proteomics datasets can be used for hypothesis generation and effective post hoc supplementation to experimental data. Overall, we highlight how the integration of proteomics, genetic, molecular, and biochemical approaches can be employed successfully to define fundamental aspects of mycobacterial pathobiology.
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spelling pubmed-77902352021-01-14 The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens Nicholson, Kathleen R. Mousseau, C. Bruce Champion, Matthew M. Champion, Patricia A. PLoS Pathog Review Mycobacterial pathogens pose a sustained threat to human health. There is a critical need for new diagnostics, therapeutics, and vaccines targeting both tuberculous and nontuberculous mycobacterial species. Understanding the basic mechanisms used by diverse mycobacterial species to cause disease will facilitate efforts to design new approaches toward detection, treatment, and prevention of mycobacterial disease. Molecular, genetic, and biochemical approaches have been widely employed to define fundamental aspects of mycobacterial physiology and virulence. The recent expansion of genetic tools in mycobacteria has further increased the accessibility of forward genetic approaches. Proteomics has also emerged as a powerful approach to further our understanding of diverse mycobacterial species. Detection of large numbers of proteins and their modifications from complex mixtures of mycobacterial proteins is now routine, with efforts of quantification of these datasets becoming more robust. In this review, we discuss the “genetic proteome,” how the power of genetics, molecular biology, and biochemistry informs and amplifies the quality of subsequent analytical approaches and maximizes the potential of hypothesis-driven mycobacterial research. Published proteomics datasets can be used for hypothesis generation and effective post hoc supplementation to experimental data. Overall, we highlight how the integration of proteomics, genetic, molecular, and biochemical approaches can be employed successfully to define fundamental aspects of mycobacterial pathobiology. Public Library of Science 2021-01-07 /pmc/articles/PMC7790235/ /pubmed/33411813 http://dx.doi.org/10.1371/journal.ppat.1009124 Text en © 2021 Nicholson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Review
Nicholson, Kathleen R.
Mousseau, C. Bruce
Champion, Matthew M.
Champion, Patricia A.
The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title_full The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title_fullStr The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title_full_unstemmed The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title_short The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens
title_sort genetic proteome: using genetics to inform the proteome of mycobacterial pathogens
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790235/
https://www.ncbi.nlm.nih.gov/pubmed/33411813
http://dx.doi.org/10.1371/journal.ppat.1009124
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